LOCUS       sequence-286497-        1712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3318
FEATURES             Location/Qualifiers
     source          1..1712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1544..1568)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1576..1617
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cctgggctct gaagctgcct
       61 ctggccgacg aagtgattga atccgggttg gtgcaggact ttgatgctag cctgtccggg
      121 atcggccagg aactgggtgc tggtgcctat agcatgagtg atgtccttgc attgcccatt
      181 tttaagcaag aagagtcgag tttgcctcct gataatgaga ataaaatcct gccttttcaa
      241 tatgtgcttt gtgctgctac ctctccagca gtgaaactcc atgatgaaac cctaacgtat
      301 ctcaatcaag gacagtctta tgaaattcga atgctagaca ataggaaact tggagaactt
      361 ccagaaatta atggcaaatt ggtgaagagt atattccgtg tggtgttcca tgacagaagg
      421 cttcagtaca ctgagcatca gcagctagag ggctggaggt ggaaccgacc tggagacaga
      481 attcttgaca tagatatccc gatgtctgtg ggtataatcg atcctagggc taatccaact
      541 caactaaata cagtggagtt cctgtgggac cctgcaaaga ggacatctgt gtttattcag
      601 gtgcactgta ttagcacaga gttcactatg aggaaacatg gtggagaaaa gggggtgcca
      661 ttccgagtac aaatagatac cttcaaggag aatgaaaacg gggaatatac tgagcactta
      721 cactcggcca gctgccagat caaagttttc aagcccaaag gtgcagacag aaagcaaaaa
      781 acggataggg aaaaaatgga gaaacgaaca cctcatgaaa aggagaaata tcagccttcc
      841 tatgagacaa ccatactcac agagtgttct ccatggcccg agatcacgta tgtcaataac
      901 tccccatcac ctggcttcaa cagttcccat agcagttttt ctcttgggga aggaaatggt
      961 tcaccaaacc accagccaga gccaccccct ccagtcacag ataacctctt accaacaacc
     1021 acacctcagg aagctcagca gtggttgcat cgaaatcgtt tttctacatt cacaaggctt
     1081 ttcacaaact tctcaggggc agatttattg aaattaacta gagatgatgt gatccaaatc
     1141 tgtggccctg cagatggaat cagacttttt aatgcattaa aaggccggat ggtgcgtcca
     1201 aggttaacca tttatgtttg tcaggaatca ctgcagttga gggagcagca acaacagcag
     1261 cagcaacagc agcagaagca tgaggatgga gactcaaatg gtactttctt cgtttaccat
     1321 gctatctatc tagaagaact aacagctgtt gaattgacag aaaaaattgc tcagcttttc
     1381 agcatttccc cttgccagat cagccagatt tacaagcagg ggccaacagg aattcatgtg
     1441 ctcatcagtg atgagatgat acagaacttt caggaagaag catgttttat tctggacaca
     1501 atgaaagcag aaaccaatga tagctatcat atcatactga agtacccaac tttcttgtac
     1561 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1621 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1681 aaggacgatt tcgactaccg cgcaaactag ct
//
