LOCUS       sequence-286500-        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3319
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1526..1550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1558..1599
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgcaaattg cacatcctca
       61 tggagtctag gagaatcctg caacagacct gggttggagc tgccacggtc tatggcatcc
      121 tcagaaactc aacttggaaa tcacgacgtg gaccctgaga tttctcacgt gaacttcagg
      181 atgttcagct gcccgaagga gtcggacccc atccaggctc tgaggaaact cactgagctg
      241 tgccatctgt ggctgaggcc cgacctccac accaaagagc agatcctgga catgctggtg
      301 atggagcagt tcatgatctc catgccccag gagctccagg tcttagtcat gatgaacggt
      361 gtgcagagct gcaaagacct ggaggacctg ctacgaaata acagaagacc caagaaatgg
      421 tctgtggtca ccttccacgg aaaggaatat attgtgcagg actcagatat cgagatggct
      481 gaagccccct ccagtgtcag agatgatctg aaagacgtgt ccagccaacg ggcctcctcg
      541 gtgaaccaga tgcgtccagg ggaaggccag gcccaccgag agctgcagat cctgcccagg
      601 gtccctgcat tgtccaggag gcagggagag gactttctgc tacacaagag tattgacgta
      661 acaggtgacc caaagtctct gagacccaag cagaccttgg agaaggatct gaaggaaaac
      721 agggaagaga acccaggact gacatcccca gagcctcagc ttccaaagag tcccacagat
      781 ctggtgagag caaaggaggg gaaggacccc ccaaaaatag cctctgtgga aaatgtggat
      841 gctgacacac cttctgcctg cgttgtggag agagaagctt cgactcacag cgggaacaga
      901 ggagacgctc tgaatctgag cagtcccaaa agaagcaaac cagatgcctc ctccatttcc
      961 caagaagagc ctcaaggaga agccacacct gtgggcaaca gagaatcccc gggacaagct
     1021 gggatgaatt caattcattc cccaggccct gcgagcccag tcagtcaccc ggatggccaa
     1081 gaagccaagg cactgccgcc ctttgcatgt gacgtgtgcg agaagaggtt tacgtgtaat
     1141 tccaagctag tcatccacaa gagatcacac acaggcgaga gactctttca atgtaatctc
     1201 tgtgggaagc gcttcatgca gcttattagc ctccaatttc accagcgaac ccacactggc
     1261 gagaggccct acacgtgtga cgtctgccag aagcagttca cccagaagtc ctacttgaag
     1321 tgtcacaaga gaagccacac aggggagaag cccttcgaat gtaaagactg caagaaagtt
     1381 ttcacctaca gggggagcct gaaggagcac cagcgcatcc actccggaga gaaaccctac
     1441 aaatgttcca agtgtccaag agccttcagt cggctgaaat tgttaagacg ccaccagaaa
     1501 acacatccag aagctacttc tcagtgccca actttcttgt acaaagtggt tgatatcggt
     1561 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1621 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga cgctgccaat
     1681 cctccgatct ctag
//
