LOCUS       sequence-286506-        1388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3321
FEATURES             Location/Qualifiers
     source          1..1388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1220..1244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1252..1293
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cgggcgagga cgagcaacag
       61 gagcaaacta tcgctgagga cctggtcgtg accaagtata agatgggggg cgacatcgcc
      121 aacagggtac ttcggtcctt ggtggaagca tctagctcag gtgtgtcggt actgagcctg
      181 tgtgagaaag gtgatgccat gattatggaa gaaacaggga aaatcttcaa gaaagaaaag
      241 gaaatgaaga aaggtattgc ttttcccacc agcatttcgg taaataactg tgtatgtcac
      301 ttctcccctt tgaagagcga ccaggattat attctcaagg aaggtgactt ggtaaaaatt
      361 gaccttgggg tccatgtgga tggcttcatc gctaatgtag ctcacacttt tgtggttgat
      421 gtagctcagg ggacccaagt aacagggagg aaagcagatg ttattaaggc agctcacctt
      481 tgtgctgaag ctgccctacg cctggtcaaa cctggaaatc agaacacaca agtgacagaa
      541 gcctggaaca aagttgccca ctcatttaac tgcacgccaa tagaaggtat gctgtcacac
      601 cagttgaagc agcatgtcat cgatggagaa aaaaccatta tccagaatcc cacagaccag
      661 cagaagaagg accatgaaaa agctgaattt gaggtacatg aagtatatgc tgtggatgtt
      721 ctcgtcagct caggagaggg caaggccaag gatgcaggac agagaaccac tatttacaaa
      781 cgagacccct ctaaacagta tggactgaaa atgaaaactt cacgtgcctt cttcagtgag
      841 gtggaaaggc gttttgatgc catgccgttt actttaagag catttgaaga tgagaagaag
      901 gctcggatgg gtgtggtgga gtgcgccaaa catgaactgc tgcaaccatt taatgttctc
      961 tatgagaagg agggtgaatt tgttgcccag tttaaattta cagttctgct catgcccaat
     1021 ggccccatgc ggataaccag tggtcccttc gagcctgacc tctacaagtc tgagatggag
     1081 gtccaggatg cagagctaaa ggccctcctc cagagttctg caagtcgaaa aacccagaaa
     1141 aagaaaaaaa agaaggcctc caagactgca gagaatgcca ccagtgggga aacattagaa
     1201 gaaaatgaag ctggggactg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1261 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1321 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaagtctg aatacgaaag
     1381 ttattgtg
//
