LOCUS       sequence-286509-        1529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3322
FEATURES             Location/Qualifiers
     source          1..1529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1361..1385)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1393..1434
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctgaaagatc agatctcctt
       61 cacttcaagt ttgaaaatta tggagattca atgttacaaa aaatgaacaa attaagagaa
      121 gagaataaat tttgtgatgt tacagttctc atagatgata ttgaggtaca gggacataaa
      181 attgtgtttg ctgcaggttc ccccttctta agagaccaat ttttactgaa tgattccaga
      241 gaggtgaaaa tctccatatt acagagttcc gaagtgggga gacaattgct cttatcctgt
      301 tatagtggtg tgctggaatt ccctgagatg gaactggtaa attacttgac tgctgcaagt
      361 tttcttcaga tgagccacat tgtagaacgg tgcacacagg ccctgtggaa gtttataaag
      421 ccaaaacaac caatggatag taaagaggga tgtgaaccac agagtgcttc tccccagtca
      481 aaagaacagc agggagatgc cagaggctcc ccaaagcagg actcaccttg tattcatcca
      541 tctgaagaca gtatggatat ggaggacagt gatattcaga ttgttaaggt agaatctatt
      601 ggggatgtat cagaggttag aagtaaaaaa gatcagaacc agtttatttc ttctgaaccc
      661 actgctttac attcatcaga gccccagcac tccctgataa attcaactgt ggaaaacaga
      721 gtaagtgaaa tagaacaaaa ccatctccac aattatgccc tttcttatac aggcagtgat
      781 aacatcatca tggcctcaaa agatgtcttt ggccctaata ttcgaggtgt agacaaaggc
      841 ctacagtggc atcaccagtg cccaaagtgt accagggtgt ttcgtcacct ggagaactac
      901 gccaaccatt taaaaatgca caaactcttt atgtgtctac tctgcggcaa gactttcact
      961 cagaaaggca accttcatcg acacatgcgt gtgcatgccg gaattaaacc tttccagtgt
     1021 aaaatctgtg ggaaaacctt ttctcagaag tgttccttac aggatcatct taaccttcac
     1081 agtggagata agccccataa atgtaactat tgtgatatgg tttttgcaca taaaccagtt
     1141 ttgaggaaac accttaaaca gctgcatggc aaaaacagct ttgataatgc caatgagaga
     1201 aatgtacaag acctcacagt ggattttgat tcttttgcat gtacaacagt cacagactct
     1261 aaagggtgtc agccacaacc cgatgcaaca caggtcctgg atgcaggtaa actggcccaa
     1321 gctgtcctga acttaagaaa tgatagtact tgtgtgaatt gcccaacttt cttgtacaaa
     1381 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1441 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1501 gacgaggagc cggttctaat cgtcatggt
//
