LOCUS       sequence-286515-        2033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3324
FEATURES             Location/Qualifiers
     source          1..2033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1865..1889)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1897..1938
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt caggctggga gtcatattac
       61 aaaaccgagg gcgatgaaga agcagaggaa gaacaagaag agaaccttga agcaagtgga
      121 gactataaat attcaggaag agatagtttg atttttttgg ttgatgcctc caaggctatg
      181 tttgaatctc agagtgaaga tgagttgaca ccttttgaca tgagcatcca gtgtatccaa
      241 agtgtgtaca tcagtaagat cataagcagt gatcgagatc tcttggctgt ggtgttctat
      301 ggtaccgaga aagacaaaaa ttcagtgaat tttaaaaata tttacgtctt acaggagctg
      361 gataatccag gtgcaaaacg aattctagag cttgaccagt ttaaggggca gcagggacaa
      421 aaacgtttcc aagacatgat gggccacgga tctgactact cactcagtga agtgctgtgg
      481 gtctgtgcca acctctttag tgatgtccaa ttcaagatga gtcataagag gatcatgctg
      541 ttcaccaatg aagacaaccc ccatggcaat gacagtgcca aagccagccg ggccaggacc
      601 aaagccggtg atctccgaga tacaggcatc ttccttgact tgatgcacct gaagaaacct
      661 gggggctttg acatatcctt gttctacaga gatatcatca gcatagcaga ggatgaggac
      721 ctcagggttc actttgagga atccagcaag ctagaagacc tgttgcggaa ggttcgcgcc
      781 aaggagacca ggaagcgagc actcagcagg ttaaagctga agctcaacaa agatatagtg
      841 atctctgtgg gcatttataa tctggtccag aaggctctca agcctcctcc aataaagctc
      901 tatcgggaaa caaatgaacc agtgaaaacc aagacccgga cctttaatac aagtacaggc
      961 ggtttgcttc tgcctagcga taccaagagg tctcagatct atgggagtcg tcagattata
     1021 ctggagaaag aggaaacaga agagctaaaa cggtttgatg atccaggttt gatgctcatg
     1081 ggtttcaagc cgttggtact gctgaagaaa caccattacc tgaggccctc cctgttcgtg
     1141 tacccagagg agtcgctggt gattgggagc tcaaccctgt tcagtgctct gctcatcaag
     1201 tgtctggaga aggaggttgc agcattgtgc agatacacac cccgcaggaa catccctcct
     1261 tattttgtgg ctttggtgcc acaggaagaa gagttggatg accagaaaat tcaggtgact
     1321 cctccaggct tccagctggt ctttttaccc tttgctgatg ataaaaggaa gatgcccttt
     1381 actgaaaaaa tcatggcaac tccagagcag gtgggcaaga tgaaggctat cgttgagaag
     1441 cttcgcttca catacagaag tgacagcttt gagaaccccg tgctgcagca gcacttcagg
     1501 aacctggagg ccttggcctt ggatttgatg gagccggaac aagcagtgga cctgacattg
     1561 cccaaggttg aagcaatgaa taaaagactg ggctccttgg tggatgagtt taaggagctt
     1621 gtttacccac cagattacaa tcctgaaggg aaagttacca agagaaaaca cgataatgaa
     1681 ggttctggaa gcaaaaggcc caaggtggag tattcagaag aggagctgaa gacccacatc
     1741 agcaagggta cgctgggcaa gttcactgtg cccatgctga aagaggcctg ccgggcttac
     1801 gggctgaaga gtggtctgaa gaagcaggag ctgctggaag ccctcaccaa gcacttccag
     1861 gactgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1921 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1981 tggctttata tatcttgtgg aaaggacgac ctcgtcttgc ttaaggattc aaa
//
