LOCUS       sequence-28652-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: pCSegfpcherry
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    15..80
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(94..118)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(265..283)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     533..552
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
       61 gtaccgcggg cccgggatcc accggatcta gacacccagc tttcttgtac aaagtggaca
      121 tggtgagcaa gggcgaggag gacaacatgg ccatcatcaa ggagttcatg cgcttcaagg
      181 tgcacatgga gggctccgtg aacggccgcg agttcgagat cgagggcgag ggcgagggcc
      241 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
      301 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      361 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      421 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      481 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      541 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
      601 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgccg
      661 aggtcaagac cacctacaag gccaagaagc ccgtgcagtc cggc
//
