LOCUS       sequence-286521-        1676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3326
FEATURES             Location/Qualifiers
     source          1..1676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1508..1532)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1540..1581
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agatgagaag aatcaagcca
       61 gctgctactt ctcatgtcga agggtcaggt ggagtatcag ccaaggggaa aaggaaaccc
      121 aggcaggaag aagatgaaga ctatcgagaa tttcctcaga agaagcataa gctttatggg
      181 aggaagcaac ggcctaaaac tcagcccaat cccaaatccc aggcccgtcg tattcggaag
      241 gaaccaccag tttatgcagc aggcagtttg gaggagcaat ggtacttaga aatcgttgat
      301 aaaggcagtg tctcctgccc tacctgccag gcagtgggga ggaagaccat agagggttta
      361 aagaaacaca tggaaaactg caagcaggaa atgtttactt gtcatcattg tgggaaacaa
      421 cttcgttcac tggcagggat gaagtatcat gtcatggcaa atcataatag tttgcccatt
      481 ttgaaagccg gagatgaaat agatgagcca agtgagaggg aaaggctccg aacagttcta
      541 aagagactgg gaaagctcag gtgcatgcgt gagagttgct ccagtagctt caccagcatc
      601 atgggatatc tctaccatgt cagaaaatgt ggcaaagggg ctgcagagct ggaaaagatg
      661 accctgaaat gtcaccactg tggaaaacca tataggtcga aggctggact tgcatatcac
      721 ctgaggtcag agcatgggcc tatatccttc tttccagagt caggacagcc agagtgctta
      781 aaggagatga acctagagtc aaagagtggg ggccgagttc agagacgttc tgccaagata
      841 gctgtatacc acctacagga gctggcctct gctgaactgg ccaaggaatg gcccaagagg
      901 aaggtgcttc aggacctggt acctgatgat cgaaagttaa aatatactcg tccagggctc
      961 cctaccttca gccaggaagt actacataaa tggaagacag atatcaagaa atatcatcgt
     1021 attcagtgtc ctaaccaggg ctgtgaggct gtctacagca gtgtatctgg ccttaaagct
     1081 cacctgggct cttgtacatt gggaaacttt gtggctggaa aatacaaatg tcttctatgt
     1141 cagaaagaat ttgtgtcaga gagtggtgtc aagtatcaca tcaactccgt ccatgctgag
     1201 gactggttcg ttgtaaaccc aacaacaacc aaaagctttg aaaagctgat gaagataaag
     1261 cagcggcagc aagaagaaga aaagcggagg cagcagcaca ggagcagaag gtctctaaga
     1321 aggcggcagc agcctggcat tgagcttccc gagacagagc tgagtcttag agtagggaag
     1381 gatcagagga ggaataatga ggaactggta gtgtcagcct cctgtaagga accagagcag
     1441 gagccagtgc cagcacagtt ccagaaagta aagcccccaa agactaatca taaacgagga
     1501 aggaaatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1561 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1621 tcttggcttt atatatcttg tggaaaggac gaatgtctgt ttatccgtct ggcggc
//
