LOCUS       sequence-286524-        1298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3327
FEATURES             Location/Qualifiers
     source          1..1298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1130..1154)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1162..1203
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcccagcc agggtccggc
       61 tgcaaagcga ccacccgctg tcttgaaggg accgcgccgc ccgccatggc tcagtctgac
      121 gccgaggccc tggcaggagc tctggacaag gacgagggtc aggcctcccc atgtacgccc
      181 agcacgccat ctgtctgctc accgccctct gccgcctcct ccgtgccgtc tgcaggcaag
      241 aacatctgct ccagctgcgg cctcgagatc ctggaccgat atctgctcaa ggtcaacaac
      301 ctcatctggc acgtgcggtg cctcgagtgc tccgtgtgtc gcacgtcgct gaggcagcag
      361 aacagctgct acatcaagaa caaggagatc ttctgcaaga tggactactt cagccgattc
      421 gggaccaagt gtgcccggtg cggccgacag atctacgcca gcgactgggt gcggagagct
      481 cgcggcaacg cctaccacct ggcctgcttc gcctgcttct cgtgcaagcg ccagctgtcc
      541 actggtgagg agttcggcct ggtcgaggag aaggtgctct gccgcatcca ctacgacacc
      601 atgattgaga acctcaagag ggccgccgag aacgggaacg gcctcacgtt ggagggggca
      661 gtgccctcgg aacaggacag tcaacccaag ccggccaagc gcgcgcggac gtccttcacc
      721 gcggaacagc tgcaggttat gcaggcgcag ttcgcgcagg acaacaaccc cgacgctcag
      781 acgctgcaga agctggcgga catgacgggc ctcagccgga gagtcatcca ggtgtggttt
      841 caaaactgcc gggcgcgtca taaaaagcac acgccgcaac acccagtgcc gccctcgggg
      901 gcgcccccgt cccgccttcc ctccgccctg tccgacgaca tccactacac cccgttcagc
      961 agccccgagc gggcgcgcat ggtcaccctg cacggctaca ttgagagtca ggtacagtgc
     1021 gggcaggtgc actgccggct gccttacacc gcaccccccg tccacctcaa agccgatatg
     1081 gatgggccgc tctccaaccg gggtgagaag gtcatccttt ttcagtactt gccaactttc
     1141 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1201 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1261 tgtggaaagg acgacgatgc ggtcttcatc taaggatc
//
