LOCUS       sequence-286527-        1250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3328
FEATURES             Location/Qualifiers
     source          1..1250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1082..1106)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1114..1155
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ccacagccct ggaacctgag
       61 gaccaaaaag gacttctgat aattaaggca gaggaccatt actggggaca ggattccagc
      121 tcacaaaagt gcagtcctca caggagggaa ctctatagac aacacttcag gaagctctgc
      181 tatcaggatg cacctggacc ccgtgaagct cttacccagc tgtgggagct ctgccgtcag
      241 tggctgaggc cagaatgcca caccaaggag cagattttag acctgctggt gctagaacag
      301 ttcctgagca ttcttcctaa agacctgcaa gcatgggtgc gtgcacacca tccagagact
      361 ggagaggagg cagtgacggt actggaggat ctggagagag agcttgatga acctggaaag
      421 caggtcccag gcaattcaga aagacgggac atactcatgg acaagttggc ccccttggga
      481 aggccatatg aatcactgac tgtccagctc catcccaaaa agacccagct ggagcaggaa
      541 gctgggaaac cacaaaggaa tggtgataaa actaggacta agaatgaaga gttgttccag
      601 aaggaagata tgcccaaaga caaggaattc cttggggaga taaatgacag actgaacaaa
      661 gatactcctc agcatcctaa gtccaaagat attattgaaa atgagggcag atcagaatgg
      721 caacagaggg aaagaagacg atataaatgt gatgaatgtg ggaaaagttt cagtcatagc
      781 tcagacctta gtaaacacag gagaactcac acgggagaga agccctataa atgtgatgag
      841 tgtggaaaag ccttcattca gcgctcacat ctcattggac atcatagagt acacacggga
      901 gtaaaaccct ataaatgtaa agaatgtggg aaagacttca gtgggcgcac aggtcttatt
      961 cagcatcaga gaatccacac aggtgaaaaa ccctatgaat gtgatgagtg tggaaggcct
     1021 ttccgagtaa gttcagctct tattagacat caaagaattc ataccgcaaa taaactctac
     1081 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1141 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1201 ctttatatat cttgtggaaa ggacgaggcg gggtggtgac ggtcgcgtac
//
