LOCUS       sequence-286530-        1220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3329
FEATURES             Location/Qualifiers
     source          1..1220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1052..1076)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1084..1125
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agtatttcat ggtgcccact
       61 cagaaggtgc cctctttgca acatttcagg aaaacagaga aagaagtgat aggagggctc
      121 tgtagccttg ccaacattcc actaaccccc gagactcagc gggaccagga gcggcggatt
      181 cggcgggaga tcgccaacag caacgagcgg agacgcatgc agagcatcaa cgcgggattc
      241 cagtccctca agaccctcat cccccacaca gacggagaga agctcagcaa ggcagccatt
      301 ctccagcaga cagccgagta catcttctcc ctggagcagg agaagaccag gctcttgcag
      361 cagaacacac agctcaagcg cttcatccag gagctgagcg gctcgtcccc caagcgacgg
      421 cgggcagagg acaaggacga aggcataggc tccccggaca tctgggagga cgagaaggcg
      481 gaggacctgc ggcgggagat gattgagctg cggcagcagc tggacaagga gcgctcggtg
      541 cgcatgatgc tggaggagca ggtgcgctcg ctggaggccc acatgtaccc ggaaaagctc
      601 aaggtgattg cgcagcaggt gcagctgcag cagcagcagg aacaggtgag gctgctgcac
      661 caggagaagc tggagcggga acagcagcag ctgcggaccc agcttctgcc ccctccggcc
      721 cccacccacc accccacggt gatcgtgcca gcaccgcctc ctcctccctc ccaccacatc
      781 aatgtcgtca ccatgggccc ctcctcggtc atcaactctg tttccacatc ccggcaaaat
      841 ctggacacca tcgtgcaggc aatccagcac atcgagggca cccaggaaaa gcaggagctg
      901 gaggaggagc agcggcgagc tgtcatcgtg aagcctgtcc gcagctgccc ggaggccccc
      961 acctctgaca ccgcctccga ctccgaggcc tcagacagtg acgccatgga ccagagccgg
     1021 gaggagccgt cgggggacgg ggagcttccc tgcccaactt tcttgtacaa agtggttgat
     1081 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1141 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacaaa
     1201 cctttcccaa gcctccaatc
//
