LOCUS       sequence-286533-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3330
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(932..956)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             964..1005
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagttcac ggcgtccccc
       61 aagccccaac tctcctcccg ggccaacgcc ttctccattg ccgcgctcat gtcgagcggc
      121 ggctctaagg agaaggaggc gacggagaac acaatcaaac ccctggagca atttgtggag
      181 aagtcgtcct gtgcccagcc cctgggtgag ctgaccagcc tggatgctca tggggagttt
      241 ggtggaggca gtggcagcag cccgtcctcc tcctctctgt gcactgagcc actgatcccc
      301 accaccccca tcatccccag tgaggaaatg gccaaaattg cctgcagcct ggagaccaag
      361 gagctttggg acaaattcca tgagctgggc accgagatga tcatcaccaa gtcgggcagg
      421 aggatgtttc caaccatccg ggtgtccttt tcgggggtgg atcctgaggc caagtacata
      481 gtcctgatgg acatcgtccc tgtggacaac aagaggtacc gctacgccta ccaccggtcc
      541 tcctggctgg tggctggcaa ggccgacccg ccgttgccag ccaggctcta tgtgcatcca
      601 gattctcctt ttaccggtga gcaactactc aaacagatgg tgtcttttga aaaggtgaaa
      661 ctcaccaaca atgaactgga tcaacatggc catataattt tgaactcaat gcataagtac
      721 cagccaaggg tgcacatcat taagaagaaa gaccacacag cctcattgct caacctgaag
      781 tctgaagaat ttagaacttt catctttcca gaaacagttt ttacggcagt cactgcctac
      841 cagaatcaac tgataacgaa gctgaaaata gatagcaatc cttttgccaa aggattccgg
      901 gattcctcca ggctcactga cattgagagg ttgccaactt tcttgtacaa agtggttgat
      961 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1021 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacct
     1081 aacctaacta cgcttgaacg
//
