LOCUS       sequence-286536-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3331
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1664..1688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1696..1737
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgacggcgac catagctctc
       61 caggtcaatg gccagcaagg aggggggtcc gagccggcgg cggcggcggc agtggtggca
      121 gcgggagaca aatggaaacc tccacagggc acagactcca tcaagatgga gaacgggcag
      181 agcacagccg ccaagctggg gctgcctccc ctgacgcccg agcagcagga ggcccttcag
      241 aaggccaaga agtacgccat ggagcagagc atcaagagtg tgctggtgaa gcagaccatc
      301 gcgcaccagc agcagcagct caccaacctg cagatggcgg ctcagcggca gcgggcgctg
      361 gccatcatgt gccgcgtcta cgtgggctct atctactatg agctggggga ggacaccatc
      421 cgccaggcct ttgccccctt tggccccatc aagagcatcg acatgtcctg ggactccgtc
      481 accatgaagc acaagggctt tgccttcgtg gagtatgagg tccccgaagc tgcacagctg
      541 gccttggagc agatgaactc ggtgatgctg gggggcagga acatcaaggt gggcagaccc
      601 agcaacatag ggcaggccca gcccatcata gaccagttgg ctgaggaggc acgggccttc
      661 aaccgcatct acgtggcctc tgtgcaccag gacctctcag acgatgacat caagagcgtg
      721 tttgaggcct ttggcaagat caagtcctgc acactggccc gggaccccac aactggcaag
      781 cacaagggct acggcttcat tgagtacgag aaggcccagt cgtcccaaga tgctgtgtct
      841 tccatgaacc tctttgacct gggtggccag tacttgcggg tgggcaaggc tgtcacaccg
      901 cccatgcccc tactcacacc agccacgcct ggaggcctcc cacctgccgc tgctgtggca
      961 gctgctgcag ccactgccaa gatcacagct caggaagcag tggccggagc agcggtgctg
     1021 ggtaccctgg gcacacctgg actggtgtcc ccagcactga ccctggccca gcccctgggc
     1081 actttgcccc aggctgtcat ggctgcccag gcacctggag tcatcacagg tgtgacccca
     1141 gcccgtcctc ctatcccggt caccatcccc tcggtgggag tggtgaaccc catcctggcc
     1201 agccctccaa cgctgggtct cctggagccc aagaaggaga aggaagaaga ggagctgttt
     1261 cccgagtcag agcggccaga gatgctgagc gagcaggagc acatgagcat ctcgggcagt
     1321 agcgcccgac acatggtgat gcagaagctg ctccgcaagc aggagtctac agtgatggtt
     1381 ctgcgcaaca tggtggaccc caaggacatc gatgatgacc tggaagggga ggtgacagag
     1441 gagtgtggca agttcggggc cgtgaaccgc gtcatcatct accaagagaa acaaggcgag
     1501 gaggaggatg cagaaatcat tgtcaagatc tttgtggagt tttccatagc ctctgagact
     1561 cataaggcca tccaggccct caatggccgc tggtttgctg gccgcaaggt ggtggctgaa
     1621 gtgtacgacc aggagcgttt tgataacagt gacctctctg cgtgcccaac tttcttgtac
     1681 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1741 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1801 aaggacgaaa ctgaccgccg aaacggatac tc
//
