LOCUS       sequence-28654-e         549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: pCSegfpN5ICD
FEATURES             Location/Qualifiers
     source          1..549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    88..222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(125..144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     179..198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    261..285
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgcattggc gccgcggccg cgaattaaaa aacctcccac acctccccct gaacctgaaa
       61 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      181 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctacgtaat acgactcact
      241 atagttctag aggctcgatc accactttgt acaagaaagc tgggtctgca ggatgggctg
      301 ctgggcatta cggaacacct gctggtgcaa tagcatgctg ctttgcaggg ttggtgtttg
      361 tgggagcatg cctgcttgac tacttactgg atgcaccatg ctgatcattg gctgactgca
      421 ctgagaagat ggaggcatgc ggttgtgcca gtcaaacggc acgcttactg gacttaccat
      481 gcccatacta aggctcattt gaccggcgtt catcacgtag ttatgggggc ctgggttgac
      541 accccctcc
//
