LOCUS       sequence-286542-        1703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3333
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1535..1559)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1567..1608
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agaacgggca gagcacagcc
       61 gccaagctgg ggctgcctcc cctgacgccc gagcagcagg aggcccttca gaaggccaag
      121 aagtacgcca tggagcagag catcaagagt gtgctggtga agcagaccat cgcgcaccag
      181 cagcagcagc tcaccaacct gcagatggcg gctcagcggc agcgggcgct ggccatcatg
      241 tgccgcgtct acgtgggctc tatctactat gagctggggg aggacaccat ccgccaggcc
      301 tttgccccct ttggccccat caagagcatc gacatgtcct gggactccgt caccatgaag
      361 cacaagggct ttgccttcgt ggagtatgag gtccccgaag ctgcacagct ggccttggag
      421 cagatgaact cggtgatgct ggggggcagg aacatcaagg tgggcagacc cagcaacata
      481 gggcaggccc agcccatcat agaccagttg gctgaggagg cacgggcctt caaccgcatc
      541 tacgtggcct ctgtgcacca ggacctctca gacgatgaca tcaagagcgt gtttgaggcc
      601 tttggcaaga tcaagtcctg cacactggcc cgggacccca caactggcaa gcacaagggc
      661 tacggcttca ttgagtacga gaaggcccag tcgtcccaag atgctgtgtc ttccatgaac
      721 ctctttgacc tgggtggcca gtacttgcgg gtgggcaagg ctgtcacacc gcccatgccc
      781 ctactcacac cagccacgcc tggaggcctc ccacctgccg ctgctgtggc agctgctgca
      841 gccactgcca agatcacagc tcaggaagca gtggccggag cagcggtgct gggtaccctg
      901 ggcacacctg gactggtgtc cccagcactg accctggccc agcccctggg cactttgccc
      961 caggctgtca tggctgccca ggcacctgga gtcatcacag gtgtgacccc agcccgtcct
     1021 cctatcccgg tcaccatccc ctcggtggga gtggtgaacc ccatcctggc cagccctcca
     1081 acgctgggtc tcctggagcc caagaaggag aaggaagaag aggagctgtt tcccgagtca
     1141 gagcggccag agatgctgag cgagcaggag cacatgagca tctcgggcag tagcgcccga
     1201 cacatggtga tgcagaagct gctccgcaag caggagtcta cagtgatggt tctgcgcaac
     1261 atggtggacc ccaaggacat cgatgatgac ctggaagggg aggtgacaga ggagtgtggc
     1321 aagttcgggg ccgtgaaccg cgtcatcatc taccaagaga aacaaggcga ggaggaggat
     1381 gcagaaatca ttgtcaagat ctttgtggag ttttccatag cctctgagac tcataaggcc
     1441 atccaggccc tcaatggccg ctggtttgct ggccgcaagg tggtggctga agtgtacgac
     1501 caggagcgtt ttgataacag tgacctctct gcgtgcccaa ctttcttgta caaagtggtt
     1561 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1621 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1681 tccggcttga aggtggcctc tta
//
