LOCUS       sequence-286545-        1646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3334
FEATURES             Location/Qualifiers
     source          1..1646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1478..1502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1510..1551
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg caggtggggg cggcgacctg
       61 agcaccagga ggctgaatga atgtatttca ccagtagcaa atgagatgaa ccatcttcct
      121 gcacacagcc acgatttgca aaggatgttc acggaagacc agggtgtaga tgacaggctg
      181 ctctatgaca ttgtattcaa gcacttcaaa agaaataagg tggagatttc aaatgcaata
      241 aaaaagacat ttccattcct cgagggcctc cgtgatcgtg atctcatcac aaataaaatg
      301 tttgaagatt ctcaagattc ttgtagaaac ctggtccctg tacagagagt ggtgtacaat
      361 gttcttagtg aactggagaa gacatttaac ctgccagttc tggaagcact gttcagcgat
      421 gtcaacatgc aggaataccc cgatttaatt cacatttata aaggctttga aaatgtaatc
      481 catgacaaat tgcctctcca agaaagtgaa gaagaagaga gggaggagag gtctggcctc
      541 caactaagtc ttgaacaagg aactggtgaa aactcttttc gaagcctgac ttggccacct
      601 tcgggttccc catctcatgc tggtacaacc ccacctgaaa atggactctc agagcacccc
      661 tgtgaaacag aacagataaa tgcaaagaga aaagatacaa ccagtgacaa agatgattcg
      721 ctaggaagcc aacaaacaaa tgaacaatgt gctcaaaagg ctgagccaac agagtcctgc
      781 gaacaaattg ctgtccaagt gaataatggg gatgctggaa gggagatgcc ctgcccgttg
      841 ccctgtgatg aagaaagccc agaggcagag ctacacaacc atggaatcca aattaattcc
      901 tgttctgtgc gactggtgga tataaaaaag gaaaagccat tttctaattc aaaagttgag
      961 tgccaagccc aagcaagaac tcatcataac caggcatctg acataatagt catcagcagt
     1021 gaggactctg aaggatccac tgacgttgat gagcccttag aagtcttcat ctcagcaccg
     1081 agaagtgagc ctgtgatcaa taatgacaac cctttagaat caaatgatga aaaggagggc
     1141 caagaagcca cttgctcacg accccagatt gtaccagagc ccatggattt cagaaaatta
     1201 tctacattca gagaaagttt taagaaaaga gtgataggac aagaccacga cttttcagaa
     1261 tccagtgagg aggaggcgcc cgcagaagcc tcaagcgggg cactgagaag caagcatggt
     1321 gagaaggctc ctatgacttc tagaagtaca tctacttgga gaatacccag caggaagaga
     1381 cgtttcagca gtagtgactt ttcagacctg agtaatggag aagagcttca ggaaacctgc
     1441 agctcatccc taagaagagg gtcaggtaaa gaagattacc caactttctt gtacaaagtg
     1501 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1561 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1621 gaagacgttt catagcctac agaggc
//
