LOCUS       sequence-286548-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3335
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1342..1383
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgctgagtc tgatgttctg
       61 catttccagt ttgaacagca aggagatgtg gtcttgcaga aaatgaatct tttgagacag
      121 cagaatttat tttgtgatgt atcaatttac attaatgaca ctgagttcca ggggcacaag
      181 gtgattttgg ctgcttgctc cacttttatg agagatcagt ttttactcac acagtcaaaa
      241 catgtcagaa tcaccatctt acagagtgca gaagttggca gaaaattgtt actgtcttgc
      301 tatactggag cacttgaagt taaaaggaaa gagcttttga aatacttgac tgctgccagt
      361 taccttcaga tggttcacat tgtggaaaag tgcacagaag ctttgtcaaa gtatctggaa
      421 attgatcttt ctatgaaaaa caacaaccaa cacactgacc tgtgtcagtc ttctgatcct
      481 gatgttaaga atgaagatga aaattctgat aaagactgtg agataattga aatttcagaa
      541 gatagtcctg taaacataga tttccatgtt aaagaagagg aaagcaatgc tttgcagtct
      601 acagtagaga gtctgacatc agagagaaag gaaatgaagt caccagagct gtctacagta
      661 gacataggtt ttaaagacaa tgaaatttgt atccttcatg tagaatccat cagtacagct
      721 ggtgtcgaaa atgggcagtt ttcacagcct tgtacctctt caaaagcaag catgtatttc
      781 tctgaaacac agcattcatt gatcaattct acagttgaga gcagagtggc tgaagttcct
      841 gggaatcaag atcagggctt attttgtgag aatactgaag gaagttatgg tacagtgagt
      901 gagattcaga atctggagga aggttattca ctgaggcacc agtgccccag gtgtcctcga
      961 ggctttcttc atgttgaaaa ctatctgcgc caccttaaaa tgcataaact attcttatgc
     1021 ttacagtgcg gaaaaacatt tacacagaag aaaaatctca accgacacat ccgaggacac
     1081 atgggcatac ggccctttca gtgtactgtg tgcttgaaga catttactgc caaaagcaca
     1141 cttcaggacc acttgaacat acacagtggg gatcggccat acaaatgcca ctgttgtgat
     1201 atggatttca agcacaagtc tgctctcaaa aagcacttaa cctctgtcca tggcagaagc
     1261 agtggtgaaa aactatctag gcctgatctc aaaaggcaaa gtctactata cccaactctc
     1321 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1381 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1441 tgtggaaagg acgagcatta cagtcgttgc caaagcta
//
