LOCUS       sequence-286551-        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3336
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1121..1145)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1153..1194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agaaaggtgg gaacatacaa
       61 ttggagattc ctgacttcag caactctgtc ctgagccatc taaaccagtt gcgcatgcag
      121 ggccgtctct gtgatattgt ggtcaatgtg caaggacaag cttttcgggc tcacaaagtg
      181 gtgctggctg ccagctcccc ctatttccgg gatcacatgt ccttgaatga gatgagtaca
      241 gtctccattt cagtcatcaa gaaccctact gtttttgaac agctcctttc tttctgttac
      301 acagggcgga tatgcctgca actggcagat atcatcagct acctaacagc tgccagtttt
      361 ctgcaaatgc agcatattat agacaaatgt acacagatcc tggagggcat tcatttcaaa
      421 attaatgtgg ctgaggttga agcagaatta agtcaaacaa ggacaaagca tcaagagaga
      481 cctccagagt ctcacagggt tacaccaaat ctcaaccgct cccttagccc acgacataat
      541 accccaaagg gaaaccggcg aggtcaggtt agtgctgtgc tggatatcag agagctaagt
      601 cctcctgagg agtccaccag ccctcagatc attgaaccaa gttctgatgt agagagccgg
      661 gagcccattc ttcggatcaa ccgagcagga cagtggtatg tggagacagg agtggcggac
      721 cgtgggggtc ggagtgatga tgaagttaga gttcttggag cagtacacat caaaactgaa
      781 aatctggagg agtggcttgg gcctgagaat cagccttctg gagaagatgg gagtagtgca
      841 gaggaagtaa cagccatggt gattgatacc acaggccatg gttctgtagg acaggaaaat
      901 tatactttag ggtcttcagg agccaaggtg gctcggccaa caagcagtga agttgacaga
      961 tttagcccct ccggcagtgt tgttcccttg acagagagac acagagccag aagtgagtct
     1021 cctgggagaa tggatgagcc taagcaaccc agctcccagg tatggagttg tggatttaga
     1081 actgctttgg tggttggagg aattgctact gtgtatgaat acccaacttt cttgtacaaa
     1141 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1201 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgagcaaa gcgcccatac atgcataac
//
