LOCUS       sequence-286554-        1652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3337
FEATURES             Location/Qualifiers
     source          1..1652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1484..1508)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1516..1557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgcagacgca gggcacccgg
       61 aggaaagtct gttactacta cgacggggat gttggaaatt actattatgg acaaggccac
      121 ccaatgaagc ctcaccgaat ccgcatgact cataatttgc tgctcaacta tggtctctac
      181 cgaaaaatgg aaatctatcg ccctcacaaa gccaatgctg aggagatgac caagtaccac
      241 agcgatgact acattaaatt cttgcgctcc atccgtccag ataacatgtc ggagtacagc
      301 aagcagatgc agagattcaa cgttggtgag gactgtccag tattcgatgg cctgtttgag
      361 ttctgtcagt tgtctactgg tggttctgtg gcaagtgctg tgaaacttaa taagcagcag
      421 acggacatcg ctgtgaattg ggctgggggc ctgcaccatg caaagaagtc cgaggcatct
      481 ggcttctgtt acgtcaatga tatcgtcttg gccatcctgg aactgctaaa gtatcaccag
      541 agggtgctgt acattgacat tgatattcac catggtgacg gcgtggaaga ggccttctac
      601 accacggacc gggtcatgac tgtgtccttt cataagtatg gagagtactt cccaggaact
      661 ggggacctac gggatatcgg ggctggcaaa ggcaagtatt atgctgttaa ctacccgctc
      721 cgagacggga ttgatgacga gtcctatgag gccattttca agccggtcat gtccaaagta
      781 atggagatgt tccagcctag tgcggtggtc ttacagtgtg gctcagactc cctatctggg
      841 gatcggttag gttgcttcaa tctaactatc aaaggacacg ccaagtgtgt ggaatttgtc
      901 aagagcttta acctgcctat gctgatgctg ggaggcggtg gttacaccat tcgtaacgtt
      961 gcccggtgct ggacatatga gacagctgtg gccctggata cggagatccc taatgagctt
     1021 ccatacaatg actactttga atactttgga ccagatttca agctccacat cagtccttcc
     1081 aatatgacta accagaacac gaatgagtac ctggagaaga tcaaacagcg actgtttgag
     1141 aaccttagaa tgctgccgca cgcacctggg gtccaaatgc aggcgattcc tgaggacgcc
     1201 atccctgagg agagtggcga tgaggacgaa gacgaccctg acaagcgcat ctcgatctgc
     1261 tcctctgaca aacgaattgc ctgtgaggaa gagttctccg attctgaaga ggagggagag
     1321 gggggccgca agaactcttc caacttcaaa aaagccaaga gagtcaaaac agaggatgaa
     1381 aaagagaaag acccagagga gaagaaagaa gtcaccgaag aggagaaaac caaggaggag
     1441 aagccagaag ccaaaggggt caaggaggag gtcaagttgg cctgcccaac tttcttgtac
     1501 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1561 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1621 aaggacgatc acagttgctg cgacatacga ac
//
