LOCUS       sequence-286557-        1658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3338
FEATURES             Location/Qualifiers
     source          1..1658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1490..1514)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1522..1563
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggcgcagac gcagggcacc
       61 cggaggaaag tctgttacta ctacgacggg gatgttggaa attactatta tggacaaggc
      121 cacccaatga agcctcaccg aatccgcatg actcataatt tgctgctcaa ctatggtctc
      181 taccgaaaaa tggaaatcta tcgccctcac aaagccaatg ctgaggagat gaccaagtac
      241 cacagcgatg actacattaa attcttgcgc tccatccgtc cagataacat gtcggagtac
      301 agcaagcaga tgcagagatt caacgttggt gaggactgtc cagtattcga tggcctgttt
      361 gagttctgtc agttgtctac tggtggttct gtggcaagtg ctgtgaaact taataagcag
      421 cagacggaca tcgctgtgaa ttgggctggg ggcctgcacc atgcaaagaa gtccgaggca
      481 tctggcttct gttacgtcaa tgatatcgtc ttggccatcc tggaactgct aaagtatcac
      541 cagagggtgc tgtacattga cattgatatt caccatggtg acggcgtgga agaggccttc
      601 tacaccacgg accgggtcat gactgtgtcc tttcataagt atggagagta cttcccagga
      661 actggggacc tacgggatat cggggctggc aaaggcaagt attatgctgt taactacccg
      721 ctccgagacg ggattgatga cgagtcctat gaggccattt tcaagccggt catgtccaaa
      781 gtaatggaga tgttccagcc tagtgcggtg gtcttacagt gtggctcaga ctccctatct
      841 ggggatcggt taggttgctt caatctaact atcaaaggac acgccaagtg tgtggaattt
      901 gtcaagagct ttaacctgcc tatgctgatg ctgggaggcg gtggttacac cattcgtaac
      961 gttgcccggt gctggacata tgagacagct gtggccctgg atacggagat ccctaatgag
     1021 cttccataca atgactactt tgaatacttt ggaccagatt tcaagctcca catcagtcct
     1081 tccaatatga ctaaccagaa cacgaatgag tacctggaga agatcaaaca gcgactgttt
     1141 gagaacctta gaatgctgcc gcacgcacct ggggtccaaa tgcaggcgat tcctgaggac
     1201 gccatccctg aggagagtgg cgatgaggac gaagacgacc ctgacaagcg catctcgatc
     1261 tgctcctctg acaaacgaat tgcctgtgag gaagagttct ccgattctga agaggaggga
     1321 gaggggggcc gcaagaactc ttccaacttc aaaaaagcca agagagtcaa aacagaggat
     1381 gaaaaagaga aagacccaga ggagaagaaa gaagtcaccg aagaggagaa aaccaaggag
     1441 gagaagccag aagccaaagg ggtcaaggag gaggtcaagt tggcctgaga cccagctttc
     1501 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1561 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1621 tgtggaaagg acgattatgg aagctaatac caattgta
//
