LOCUS       sequence-286560-        1490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3339
FEATURES             Location/Qualifiers
     source          1..1490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1322..1346)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1354..1395
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccaagaccgt ggcctatttc
       61 tacgaccccg acgtgggcaa cttccactac ggagctggac accctatgaa gccccatcgc
      121 ctggcattga cccatagcct ggtcctgcat tacggtctct ataagaagat gatcgtcttc
      181 aagccatacc aggcctccca gcatgacatg tgccgcttcc actccgagga ctacattgac
      241 ttcctgcaga gagtcagccc caccaatatg caaggcttca ccaagagtct taatgccttc
      301 aacgtaggcg atgactgccc agtgtttccc gggctctttg agttctgctc gcgttacaca
      361 ggcgcatctc tgcaaggagc aacccagctg aacaacaaga tctgtgatat tgccattaac
      421 tgggctggtg gtctgcacca tgccaagaag tttgaggcct ctggcttctg ctatgtcaac
      481 gacattgtga ttggcatcct ggagctgctc aagtaccacc ctcgggtgct ctacattgac
      541 attgacatcc accatggtga cggggttcaa gaagctttct acctcactga ccgggtcatg
      601 acggtgtcct tccacaaata cggaaattac ttcttccctg gcacaggtga catgtatgaa
      661 gtcggggcag agagtggccg ctactactgt ctgaacgtgc ccctgcggga tggcattgat
      721 gaccagagtt acaagcacct tttccagccg gttatcaacc aggtagtgga cttctaccaa
      781 cccacgtgca ttgtgctcca gtgtggagct gactctctgg gctgtgatcg attgggctgc
      841 tttaacctca gcatccgagg gcatggggaa tgcgttgaat atgtcaagag cttcaatatc
      901 cctctactcg tgctgggtgg tggtggttat actgtccgaa atgttgcccg ctgctggaca
      961 tatgagacat cgctgctggt agaagaggcc attagtgagg agcttcccta tagtgaatac
     1021 ttcgagtact ttgccccaga cttcacactt catccagatg tcagcacccg catcgagaat
     1081 cagaactcac gccagtatct ggaccagatc cgccagacaa tctttgaaaa cctgaagatg
     1141 ctgaaccatg cacctagtgt ccagattcat gacgtgcctg cagacctcct gacctatgac
     1201 aggactgatg aggctgatgc agaggagagg ggtcctgagg agaactatag caggccagag
     1261 gcacccaatg agttctatga tggagaccat gacaatgaca aggaaagcga tgtggagatt
     1321 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1381 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1441 ctttatatat cttgtggaaa ggacgatacc tccataaacc gaagaatgac
//
