LOCUS       sequence-286566-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3341
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(860..884)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             892..933
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgggcagcaa agaagatgcg
       61 ggcaaggggt gtccggcggc cggtggcgtc tccagcttca ccatccagtc catcctgggc
      121 gggggcccct cggaggcacc gcgggagccc gtcggctggc cagccaggaa gcgcagcctg
      181 tccgtgtcct cggaggagga ggagccggac gacggctgga aggcgcccgc ctgcttctgc
      241 ccagaccagc acggccctaa ggagcagggc cccaagcacc atccccccat cccttttcct
      301 tgcctgggta cccccaaggg cagcggaggc tcgggcccgg gcggcttgga gcgcacgcct
      361 ttcctctctc cttcgcactc ggactttaaa gaagagaaag agaggctcct gcccgcgggc
      421 tcgccctcgc cggggtccga gcggccgcgg gacggcggcg ctgagcggca ggccggcgcg
      481 gccaagaaga agacgcgcac cgtcttttcg cgcagccagg tgtaccagct cgagtccacc
      541 ttcgacatga agcgctacct gagcagctcg gagcgcgcct gcctcgcctc cagcctgcag
      601 ctcacggaga cccaggtaaa gacttggttc cagaaccgcc gcaacaagtg gaagcggcag
      661 ctctcggctg agctggaggc ggccaacatg gcgcacgcgt cggcgcagac tctggtgagc
      721 atgccgctgg tgttccggga cagttcgctg ctgcgcgtgc cggtgccgcg ctcgctcgcc
      781 tttcccgcgc cgctctacta cccgggaagc aacctctcgg ccttacctct ctacaaccta
      841 tacaacaagc tcgactactt gccaactttc ttgtacaaag tggttgatat cggtaagcct
      901 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      961 agtatttcga tttcttggct ttatatatct tgtggaaagg acgattgatt tccagtgcga
     1021 gaagaccg
//
