LOCUS       sequence-286575-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3344
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1664..1688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1696..1737
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gagatatctt tttgtgtaag
       61 aaagtggaat caccaaagaa gaatttgaga gaatccaaac aaagggagga ggatgatgaa
      121 gatccagatc tgatctatgt tggggtggag catgtacata gagatgctga agttctcttt
      181 gtcgggatga tttcaaattc aaaaccagtc gtttcaaaca ttttgaacag agtcacccca
      241 ggctcaaatt caagaagaaa gaaaggccac ttccgtcaat atcctgctca cgtgtcgcag
      301 cctgcaaatc atgtgacctc tatggcaaaa gccatcatgc cggtttctct gtctgagggg
      361 cgatcgacag atagtcctgt cactatgaag tcttcatctg aacctggtta taaaatgagc
      421 tcaccacaag ttgtttctcc cagttcctca gactcgctcc ccccagggac tcagtgtcta
      481 gttggagcta tggtctctgg aggaggcaga aatgagagtt ctcctgattc aaagcgactt
      541 tccacttcag atataaacag cagagattcc aaaagggtta aactcaggga tggaatccca
      601 ggggtacctt ctttagctgt ggtcccttca gatatgtctt ctacaataag cacaaataca
      661 ccctcacagg ggatctgcaa ctcatcaaac catgttcaga atggagtaac atttccttgg
      721 cctgatgcta atggaaaggc acatttcaat cttacagatc cagagagagc aagtgagtct
      781 gccctggcaa tgacagacat ttcaagtcta gcaagtcaaa acaagacctt tgatcccaag
      841 aaagaaaatc ccatcgtgtt acttagcgac ttttactatg gacagcataa aggagatggg
      901 cagccggaac agaagactca caccaccttt aaatgcctca gctgcgtgaa agttctaaaa
      961 aatattaagt ttatgaatca catgaagcat catttggaat ttgagaagca gaggaacgac
     1021 agctgggaag accacaccac ctgccagcac tgccaccggc agtttcccac tcccttccag
     1081 ctacagtgtc acattgatag tgtacacatc gccatggggc cctctgctgt ctgtaaaatc
     1141 tgtgaattgt catttgaaac agatcaggtc ctcttacaac acatgaagga ccatcataag
     1201 cctggcgaaa tgccttatgt gtgccaggtt tgccattaca gatcgtcggt ctttgctgat
     1261 gtggaaacac attttagaac gtgccatgaa aacacaaaga atttgctttg tctgttttgt
     1321 ctcaaacttt tcaaaactgc aataccatac atgaatcatt gttggaggca cagcagaagg
     1381 agggtccttc agtgttccaa gtgccggcta cagtttttga cgttgaagga ggaaatagag
     1441 cacaaaacca aggaccatca aacatttaaa aagccggagc aactgcaagg gttgcctagt
     1501 gaaacaaaag ttattattca aacttcagtt cagccaggat caagtggtat ggcttccgtt
     1561 attgttagca acactgaccc tcagtcttct cctgtaaaaa ctaaaaagaa gacggctatg
     1621 aacactagag attccagact cccttgcagc aaggattcta gctgcccaac tttcttgtac
     1681 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1741 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1801 aaggacgact gttcaattgg tgactaaccc ac
//
