LOCUS       sequence-286578-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3345
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(473..497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             505..546
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tctcagtcca gccagacacc
       61 aagccgaaag gttgtgctgg ctgcaaccga aagatcaagg accggtatct tctaaaggca
      121 ctggacaaat actggcatga agactgcctg aagtgtgcct gctgtgactg tcgcttggga
      181 gaggtgggct ccaccctgta cactaaagct aatcttatcc tttgtcgcag agactatctg
      241 aggctctttg gtgtaacggg aaactgcgct gcctgtagta agctcatccc tgcctttgag
      301 atggtgatgc gtgccaagga caatgtttac cacctggact gctttgcatg tcagctttgt
      361 aatcagagat tttgtgttgg agacaaattt ttcctaaaga ataacatgat cctttgccag
      421 acggactacg aggaaggttt aatgaaagaa ggttatgcac cccaggttcg ctgcccaact
      481 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
      541 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      601 tcttgtggaa aggacgaagc gatgataccc taacgcctag g
//
