LOCUS       sequence-286584-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3347
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(980..1004)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1012..1053
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acgccttcaa ggggggcatg
       61 agcctggagc ggctgccgga ggggctccgg ccgccgccgc cgccacccca tgacatgggg
      121 cccgccttcc acctggcccg gcccgccgac ccccgcgagc cgctcgagaa ctccgccagc
      181 gagtcgtctg acacggagct gccagagaag gagcgcggcg gggaacccaa ggggcccgag
      241 gacagtggtg cgggaggcac gggctgcggc ggcgcagacg acccagccaa gaagaagaag
      301 cagcggcggc aacgtacgca cttcacaagc cagcagttgc aagagctaga ggccacgttc
      361 cagaggaacc gctaccccga catgagcatg agggaggaga tcgccgtgtg gaccaacctc
      421 accgagccgc gcgtgcgggt ctggttcaag aaccggcgag ccaagtggcg taagcgcgag
      481 cgtaaccagc agctggacct gtgcaagggt ggctacgtgc cgcagttcag cggcctagtg
      541 cagccctacg aggacgtgta cgccgccggc tactcctaca acaactgggc cgccaagagc
      601 ctggcgccag cgccgctctc caccaagagc ttcaccttct tcaactccat gagcccgctg
      661 tcgtcgcagt ccatgttctc agcacccagc tccatctcct ccatgaccat gccgtccagc
      721 atgggcccag gcgccgtgcc tggcatgccc aactcgggcc tcaacaacat caacaacctc
      781 accggctcct cgctcaactc ggccatgtcg ccgggcgctt gcccgtacgg cactcccgcc
      841 tcgccctaca gcgtctaccg ggacacgtgc aactcgagcc tagccagcct gcggctcaag
      901 tccaaacagc actcgtcgtt tggctacggc ggcctgcagg gcccggcctc gggcctcaac
      961 gcgtgccagt acaacagctg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1021 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1081 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacctt agactcttcc
     1141 gctccctt
//
