LOCUS       sequence-286587-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3348
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1010..1034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1042..1083
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga actgcatgaa aggcccgctt
       61 cacttggagc accgagcagc ggggaccaag ctgtcggccg tctcctcatc ttcctgtcac
      121 catccccagc cgttagccat ggcttcggtt ctggctcccg gtcagccccg gtcgctggac
      181 tcctccaagc acaggctgga ggtgcacacc atctccgaca cctccagccc ggaggccgca
      241 gagaaagata aaagccagca ggggaagaat gaggacgtgg gcgccgagga cccgtctaag
      301 aagaagcggc aaaggcggca gcggactcac tttaccagcc agcagctcca ggagctggag
      361 gccactttcc agaggaaccg ctacccggac atgtccacac gcgaagaaat cgctgtgtgg
      421 accaacctta cggaagcccg agtccgggtt tggttcaaga atcgtcgggc caaatggaga
      481 aagagggagc gcaaccagca ggccgagcta tgcaagaatg gcttcgggcc gcagttcaat
      541 gggctcatgc agccctacga cgacatgtac ccaggctatt cctacaacaa ctgggccgcc
      601 aagggcctta catccgcctc cctatccacc aagagcttcc ccttcttcaa ctctatgaac
      661 gtcaaccccc tgtcatcaca gagcatgttt tccccaccca actctatctc gtccatgagc
      721 atgtcgtcca gcatggtgcc ctcagcagtg acaggcgtcc cgggctccag tctcaacagc
      781 ctgaataact tgaacaacct gagtagcccg tcgctgaatt ccgcggtgcc gacgcctgcc
      841 tgtccttacg cgccgccgac tcctccgtat gtttataggg acacgtgtaa ctcgagcctg
      901 gccagcctga gactgaaagc aaagcagcac tccagcttcg gctacgccag cgtgcagaac
      961 ccggcctcca acctgagtgc ttgccagtat gcagtggacc ggcccgtgtg cccaactttc
     1021 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1081 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1141 tgtggaaagg acgaatgtta tcagatcccc cgcaacgg
//
