LOCUS       sequence-286590-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3349
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(653..677)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             685..726
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tggggttttt gtccgcacgc
       61 caaacgggtt tggaggaccc tcttcgcctt cggagagcag agtcaacacg gagagttttg
      121 ggactggaat taaataaaga cagagatgtt gaaagaatcc acggcggtgg aattaacacc
      181 cttgacattg aacctgttga agggagatac atgttatcag gtggttcaga tggtgtgatt
      241 gtactttatg accttgagaa ctccagcaga caatcttatt acacatgtaa agcagtgtgt
      301 tccattggca gagatcatcc tgatgttcac agatacagtg tggagactgt acagtggtat
      361 cctcatgaca ctggcatgtt cacatcaagc tcatttgata aaactctgaa agtatgggat
      421 acaaatacat tacaaactgc agatgtattt aattttgagg aaacagttta cagtcatcat
      481 atgtctccag tctccaccaa gcactgtttg gtagcagttg gtactagagg acccaaagta
      541 caactttgtg acttgaagtc tggatcctgt tctcacattc tacagggtat ttttatttta
      601 tttcaaacgg caactacttt gagtaaacga ttcaataaaa agaaacgtta ctacccaact
      661 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
      721 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      781 tcttgtggaa aggacgatgc agccactttt ttaggcacct t
//
