LOCUS       sequence-286593-        1697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3350
FEATURES             Location/Qualifiers
     source          1..1697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1529..1553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1561..1602
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcttccat ggaggagatt
       61 caggtggagc tgcaatgtgc tgacctctgg aagcggttcc atgatattgg aactgaaatg
      121 atcatcacca aagcaggcag gaggatgttt cctgccatga gagtgaaaat cactggccta
      181 gatccacatc agcagtacta catagcaatg gacattgtgc ctgtggacaa taaaagatac
      241 agatatgtgt atcatagctc caagtggatg gtggctggca atgctgattc ccctgtgccc
      301 ccaagagttt atatacaccc tgattctcta gcttctggag acacctggat gagacaggtg
      361 gtcagttttg acaaactcaa gcttaccaac aatgagttgg atgatcaagg acatatcatt
      421 ctgcactcta tgcacaaata ccagcctcga gttcatgtga ttcgcaaaga cttcagcagt
      481 gacctttcac ccactaagcc tgttcctgtt ggggatgggg tgaaaacgtt caactttcct
      541 gagactgtgt tcaccacagt tacggcctat cagaatcagc agattaccag attaaaaatt
      601 gaccgaaacc cttttgctaa aggattcaga gattctggga gaaacagaac tggacttgaa
      661 gccatcatgg agacatatgc attctggaga cctcctgtgc gcacactcac cttcgaagac
      721 ttcaccacca tgcagaagca gcaaggaggc agcacaggca cttccccaac cacctccagc
      781 actgggacac catccccttc ggcttcttct catcttttat ctccatcctg ttctcctcca
      841 acttttcatc tggcccccaa cactttcaat gtgggctgcc gagaaagcca gctgtgtaat
      901 ctaaacctct ctgattatcc accatgtgcc cgaagcaaca tggctgcctt gcagagctac
      961 ccagggctga gtgacagtgg ctacaacagg cttcagagtg gcaccacttc agccactcag
     1021 ccctctgaaa ccttcatgcc tcagaggact ccatccctga tctcaggaat accaactcct
     1081 ccctcgttgc ctggcaacag caagatggaa gcctacggtg gccagctggg gtcctttccc
     1141 acttcccagt ttcagtatgt catgcaggca ggcaatgctg cctccagctc ctcatcacca
     1201 cacatgttcg ggggcagcca catgcagcag agctcctaca atgccttctc ccttcacaac
     1261 ccttacaacc tgtatggata caatttcccc acttccccta ggctagctgc aagcccggaa
     1321 aaactgagcg cctctcaaag cactttactc tgttcttctc cttccaacgg ggcctttgga
     1381 gagaggcagt acctgccgtc agggatggag cacagcatgc acatgattag tccttcaccc
     1441 aataaccaac aggcaaccaa cacttgtgat ggccggcagt atggggcagt tccaggctcc
     1501 tcctcccaga tgtccgtgca catggttttg ccaactttct tgtacaaagt ggttgatatc
     1561 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1621 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatttggct
     1681 ttttcaccgc ctactga
//
