LOCUS       sequence-286596-        2081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3351
FEATURES             Location/Qualifiers
     source          1..2081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1913..1937)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1945..1986
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagaccagag aatggacatt
       61 tcttcaacca tcagtgattt catgtccccg ggccccaccg acctgctttc cagctctctt
      121 ggtaccagtg gtgtggattg caaccgcaaa cggaaaggca gctccactga ctaccaagaa
      181 agcatggaca cagacaaaga tgaccctcat ggaaggttag aatatacaga acaccaagga
      241 aggataaaaa atgcaaggga agctcacagt cagattgaaa agcggcgtcg ggataaaatg
      301 aacagtttta tagatgaatt ggcttctttg gtaccaacat gcaacgcaat gtccaggaaa
      361 ttagataaac ttactgtgct aaggatggct gttcagcaca tgaaaacatt aagaggtgcc
      421 accaatccat acacagaagc aaactacaaa ccaacttttc tatcagacga tgaattgaaa
      481 cacctcattc tcagggcagc agatggattt ttgtttgtcg taggatgtga ccgagggaag
      541 atactctttg tctcagagtc tgtcttcaag atcctcaact acagccagaa tgatctgatt
      601 ggtcagagtt tgtttgacta cctgcatcct aaagatattg ccaaagtcaa ggagcagctc
      661 tcctcctctg acaccgcacc ccgggagcgg ctcatagatg caaaaactgg acttccagtt
      721 aaaacagata taacccctgg gccatctcga ttatgttctg gagcacgacg ttctttcttc
      781 tgtaggatga agtgtaacag gccttcagta aaggttgaag acaaggactt cccctctacc
      841 tgctcaaaga aaaaagatcg aaaaagcttc tgcacaatcc acagcacagg ctatttgaaa
      901 agctggccac ccacaaagat ggggctggat gaagacaacg aaccagacaa tgaggggtgt
      961 aacctcagct gcctcgtcgc aattggacga ctgcattctc atgtagttcc acaaccagtg
     1021 aacggggaaa tcagggtgaa atctatggaa tatgtttctc ggcacgcgat agatggaaag
     1081 tttgtttttg tagaccagag ggcaacagct attttggcat atttaccaca agaacttcta
     1141 ggcacatcgt gttatgaata ttttcaccaa gatgacatag gacatcttgc agaatgtcat
     1201 aggcaagttt tacagacgag agaaaaaatt acaactaatt gctataaatt taaaatcaaa
     1261 gatggttctt ttatcacact acggagtcga tggttcagtt tcatgaaccc ttggaccaag
     1321 gaagtagaat atattgtctc aactaacact gttgttttag ccaacgtcct ggaaggcggg
     1381 gacccaacct tcccacagct cacagcatcc ccccacagca tggacagcat gctgccctct
     1441 ggagaaggtg gcccaaagag gacccacccc actgttccag ggattccagg gggaacccgg
     1501 gctggggcag gaaaaatagg ccgaatgatt gctgaggaaa tcatggaaat ccacaggata
     1561 agagggtcat cgccttctag ctgtggctcc agcccattga acatcacgag tacgcctccc
     1621 cctgatgcct cttctccagg aggcaagaag attttaaatg gagggactcc agacattcct
     1681 tccagtggcc tactatcagg ccaggctcag gagaacccag gttatccata ttctgatagt
     1741 tcttctattc ttggtgagaa cccccacata ggtatagaca tgattgacaa cgaccaagga
     1801 tcaagtagtc ccagtaatga tgaggcagca atggctgtca tcatgagcct cttggaagca
     1861 gatgctggac tgggtggccc tgttgacttt agtgacttgc catggccgct gtacccaact
     1921 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1981 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2041 tcttgtggaa aggacgactc tctcttccca acttcctagg c
//
