LOCUS       sequence-286599-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3352
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(593..617)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             625..666
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctcccagcct tcaggaaggc
       61 gctcagctcg gggaaaacaa accctcaact tgctcctttt caattgagag aatcttagga
      121 ctggaccaga agaaagactg tgttccatta atgaaacccc acaggccctg ggcagacacc
      181 tgcagctcat cagggaaaga tggtaactta tgtctacatg tcccaaatcc tcccagtggg
      241 atttcattcc ctagcgtggt ggatcaccca atgccagaag aaagagcttc gaaatatgaa
      301 aattactttt cagcctcaga aagactgtct ttgaaaagag agttgagttg gtatagaggc
      361 cgaagaccaa gaactgcttt tactcaaaac cagattgaag tgttagaaaa tgtctttaga
      421 gtaaactgct atcctggtat cgatattaga gaagacttag ctcaaaaatt gaatctagag
      481 gaagacagaa tccagatttg gtttcaaaat cggcgtgcaa aactgaaaag gtcccataga
      541 gaatcacagt ttctaatggc gaaaaaaaat ttcaacacaa atctgctgga atacccaact
      601 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
      661 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      721 tcttgtggaa aggacgaaca ccagcattac cctcgctcat t
//
