LOCUS       sequence-286602-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3353
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(857..881)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             889..930
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ttggatttcc aacagctacc
       61 ctgctggact gtcatggaag atatgcccag aatgtagcgt tcttcaatgt gatgactgaa
      121 gcccaccaca aatatgacca ctctgaggct acaggatcct caagctggga tatccaaaat
      181 tctttcagaa gagagaagct ggaacaaaaa tccccagatt cgaagacact acaggaagat
      241 tcacctggag tgagacaaag ggtctatgag tgccaggagt gtggaaaatc cttccggcaa
      301 aaaggtagtc taacgttaca tgagagaatc cacactggtc aaaagccttt tgagtgcacc
      361 cactgtggaa aaagcttcag ggccaaaggc aatcttgtta cacatcaacg gatacacacg
      421 ggagagaagc cttatcagtg caaggagtgt gggaaaagct tcagtcaacg aggtagtctc
      481 gctgtccacg agagactcca cactggacag aaaccctacg agtgtgctat ttgtcagaga
      541 agcttcagga atcagagtaa ccttgctgtt cacaggagag ttcacagtgg tgagaagccc
      601 tatagatgtg atcagtgtgg aaaagccttc agtcagaaag gaagcttaat tgttcacatc
      661 agagtccaca caggcctgaa gccctatgcc tgtacccagt gcaggaagag tttccacacc
      721 agggggaatt gtattctgca tggcaaaatc cacacaggag agacacccta tctgtgcggc
      781 cagtgtggaa aaagcttcac ccagagaggg agtctggctg tgcaccagcg aagctgctca
      841 cagaggctca ccctttgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      901 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      961 atttcgattt cttggcttta tatatcttgt ggaaaggacg agttcaccgt tgtgtcccct
     1021 agatg
//
