LOCUS       sequence-286605-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3354
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             733..774
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggcggcggt tcggatgaac
       61 atccagatgc tgctggaggc ggccgactat ctggagcggc gggagagaga agctgaacat
      121 ggttatgcct ccatgttacc atacaataac aaggacagag atgccttaaa acggaggaac
      181 aaatccaaaa agaataacag cagtagcaga tcaactcaca atgaaatgga gaagaataga
      241 cgggctcatc ttcgcttgtg cctggagaag ttgaaggggc tggtgccact gggacccgaa
      301 tcaagtcgac acactacgtt gagtttatta acaaaagcca aattgcacat aaagaaactt
      361 gaagattgtg acagaaaagc cgttcaccaa atcgaccagc ttcagcgaga gcagcgacac
      421 ctgaagaggc agctggagaa gctgggcatt gagaggatcc ggatggacag catcggctcc
      481 accgtctcct cggagcgctc cgactccgac agggaagaaa tcgacgttga cgtggagagc
      541 acggactatc tcacaggtga tctggactgg agcagcagca gtgtgagcga ctctgacgag
      601 cggggcagca tgcagagcct cggcagtgat gagggctatt ccagcaccag catcaagaga
      661 ataaagctgc aggacagtca caaggcgtgt cttggtctct acccaacttt cttgtacaaa
      721 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
      781 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      841 gacgatttac acctccgtta accaacccc
//
