LOCUS       sequence-286608-        1499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3355
FEATURES             Location/Qualifiers
     source          1..1499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1331..1355)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1363..1404
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gtccagtaat tggaatgact
       61 ccagataaga gagctgaaac cccaggagct gaaaagattg caggattaag ccagatttac
      121 aaaatgggaa gcttgcctga agctgttgat gctgccaggc cgaaggccac tctagtggac
      181 agtgagtcag cagatgatga actcacaaac ttgaactggc ttcatgaaag cactaatctt
      241 ctaacaaact tcagcctcgg aagtgagggt cttccaattg ttagtccatt gtatgacata
      301 gagggagatg atgtgccatc ctttggacca gcttgctacc agaacccaga aaaaaaatca
      361 gcgacttcaa agcccccata ctcctttagt cttctcattt atatggccat tgagcactct
      421 ccaaataaat gtttgcctgt caaagaaatt tatagctgga ttctggacca ttttccatat
      481 tttgctactg caccaacagg ctggaagaat tctgttcgac ataatctgtc cctgaataaa
      541 tgttttcaga aagtggaaag aagccatggc aaggttaatg gaaaaggttc cttatggtgt
      601 gttgatccgg aatataaacc caatcttatc caggcactga agaagcaacc tttttcttca
      661 gcatcttcac aaaatggttc tttatcacct cactatttaa gctctgtaat caagcagaac
      721 caggtgcgaa acctcaaaga atctgatatt gatgctgctg ctgcaatgat gcttttaaat
      781 acttctatag aacaaggaat tttagaatgt gagaagcctc ttcctcttaa aacagcattg
      841 caaaaaaaga ggagttacgg caatgcattt catcatccca gtgctgtacg attacaagag
      901 agtgattctt tagccaccag cattgatcca aaagaagatc acaattacag tgcaagtagc
      961 atggcagcac agcgttgtgc atccaggtct agcgtgtctt ctctgtcttc tgtggatgag
     1021 gtatatgaat ttatcccaaa gaatagtcac gtgggaagtg atggcagtga aggatttcac
     1081 agtgaagaag atacagacgt tgattatgaa gatgatcctc ttggagacag tggctatgca
     1141 tcacagcctt gtgcaaaaat ctctgaaaaa gggcagtcag gcaaaaagat gcgaaaacag
     1201 acatgtcaag aaattgatga ggagctcaaa gaggcagctg gatctctgct ccaccttgct
     1261 ggaattcgta catgtttagg ttccctaata agtactgcaa agacacaaaa tcaaaagcaa
     1321 cggaaaaaat acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1381 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1441 atttcttggc tttatatatc ttgtggaaag gacgaactca ccctggaggg ttactcggc
//
