LOCUS       sequence-286614-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3357
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1568..1592)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1600..1641
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cctttcgcca tttaattgag
       61 ttcacatata cagcaaaatt aatgatacaa ggagaagaag aagccaatga tgtatggaaa
      121 gcagcagagt ttctacaaat gctagaagct atcaaagccc ttgaagtcag gaacaaagaa
      181 aactcagctc ccttagagga aaataccaca ggaaaaaatg aggccaaaaa aaggaagatt
      241 gcagaaactt caaatgttat cactgagtca ttgccatctg cagaatcaga acctgttgaa
      301 attgaggtag agattgccga aggcaccatt gaagtggaag atgaaggcat cgaaacatta
      361 gaggaagtgg cttctgccaa gcagtccgta aagtacatac agagcacagg ttcctctgat
      421 gattctgctc tagcactgtt ggcagatatt accagcaagt accgtcaagg tgacagaaaa
      481 gggcagatta aagaagatgg ctgtccatct gaccccacga gcaaacagga gcacatgaaa
      541 tcacactcca ctgagagttt caagtgtgaa atatgcaata aacgatatct tcgagagagc
      601 gcatggaaac agcacctaaa ttgttaccac cttgaagaag gtggagtcag taagaagcaa
      661 agaactggga aaaaaattca tgtatgtcag tactgtgaga aacagtttga ccattttgga
      721 cattttaaag aacatcttcg aaaacataca ggtgaaaaac cttttgaatg tccaaattgt
      781 catgaacgat ttgctagaaa tagcactctg aaatgtcacc tcactgcatg ccaaactgga
      841 gtaggggcaa aaaaaggaag gaagaagctc tacgaatgcc aggtctgcaa cagtgtgttt
      901 aacagctggg accagttcaa agatcacttg gtaatacaca ctggagataa acccaaccat
      961 tgtactttat gtgatttgtg gtttatgcaa ggaaatgaat taaggaggca tctcagtgat
     1021 gctcacaata tttcagagcg tctagtaacg gaagaagttc tttcagtaga aacacgtgtg
     1081 caaactgaac ctgtaacatc aatgactatt atagaacaag ttgggaaggt gcatgtgcta
     1141 ccattgcttc aggttcaggt ggattcagca caagtgactg tggaacaagt ccatccagat
     1201 ctgctccagg acagccaggt gcacgattca cacatgagtg agcttccaga gcaggtccaa
     1261 gtgagttatc tagaagtggg ccgaattcag actgaagaag gtactgaagt acatgtagag
     1321 gagctgcatg ttgaacgggt caatcaaatg ccagtggaag tacaaactga acttctagaa
     1381 gcagatttgg accacgtgac cccagaaatc atgaaccaag aggagagaga gtctagccaa
     1441 gcagatgctg ctgaggctgc cagggaagat cacgaagatg ctgaggattt agagaccaag
     1501 ccaacagtgg attctgaagc agaaaaggca gagaatgagg acagaacagc tctgccagtt
     1561 ttagaatgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1621 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1681 tcttggcttt atatatcttg tggaaaggac gaacaagatg gatagcatct cggttg
//
