LOCUS       sequence-286620-        2255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3359
FEATURES             Location/Qualifiers
     source          1..2255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2087..2111)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2119..2160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgtcgggccc gggctcccag
       61 gaacgggaag ggctcctgat agtgaagctg gaggaggact gcgcctggag ccaggagctg
      121 cccccacctg acccaggacc gagccccgag gcctcccact tgcgcttcag acggttccgc
      181 ttccaagagg cagctggtcc ccgggaagcc ctcagccggc tccaagagct ttgccatggg
      241 tggcttcggc ctgagatgcg cacgaaggag cagatcttgg agctgctggt gttagagcag
      301 ttcctgacca tcctgcccca ggagatccag agcagggtgc aggagctgca tccggagagc
      361 ggcgaagaag cggtgaccct tgtggaggat atgcagagag agcttgggag actgagacaa
      421 caggtcacaa accatgggcg gggaacagaa gtgcttttgg aggagccttt gcctctggaa
      481 acagcacgag agtcaccgag cttcaagctg gagccaatgg agactgagcg aagccctggc
      541 cccaggctgc aggagctgct aggccccagc ccccaaaggg acccccaggc tgtaaaggag
      601 agggcattat ctgctccctg gctttctctt tttcctcctg aagggaacat ggaagacaag
      661 gagatgactg ggccccagtt gcctgagagc ttagaggacg tggcaatgta catctcccag
      721 gaggagtggg ggcatcagga tcctagtaag agggccctct ccagggacac ggtgcaggag
      781 agttatgaga atgtggactc actggagtct cacattccca gtcaggaggt cccaggcacc
      841 caggtgggac aaggaggaaa gctatgggat cccagtgtcc agagctgcaa ggagggcctg
      901 agccccagag gcccagctcc aggagaagag aaatttgaga acctggaagg tgttccgtct
      961 gtatgctctg agaacatcca ccctcaggtg ctgcttcctg accaggcccg aggggaggtg
     1021 ccctggagtc ctgagctggg aagacctcat gaccggtcgc aaggggattg ggcgcctccc
     1081 ccagagggtg gaatggagca ggccttggca ggagcctcaa gtggcagaga actggggcga
     1141 ccgaaggaac tgcagccaaa gaaactccat ttatgtccct tgtgtggcaa aaatttctct
     1201 aacaactcaa acctaattag gcaccagaga atacatgcag ctgaaagact gtgtatgggt
     1261 gtggactgca ctgaaatctt tggtgggaac ccacgtttcc tgtcactaca cagagcacac
     1321 ctgggagagg aggcccacaa gtgccttgaa tgtgggaaat gcttcagtca gaacacccat
     1381 ctgactcgcc accaacgcac ccacacgggt gagaagccct atcagtgcaa catttgcgga
     1441 aaatgtttct cctgcaactc caacctccac aggcaccaga gaacgcacac tggggagaag
     1501 ccctacaagt gccctgagtg tggggagatc tttgctcaca gttccaacct ccttcggcac
     1561 cagagaattc acactggaga gcgaccttat aagtgtcccg agtgtgggaa aagtttctct
     1621 cggagttcac acctcgtcat tcacgaaaga actcatgaga gagagagact ttaccccttc
     1681 tctgagtgtg gggaagctgt gagtgacagc accccctttc ttacaaacca tggagcccat
     1741 aaggcagaga agaagctctt tgaatgtttg acttgtggga aaagcttccg gcagggcatg
     1801 cacctcacca gacatcagag aacacacaca ggagagaaac cgtataaatg taccctttgt
     1861 ggggaaaact tctctcatag atccaattta atcaggcacc agagaatcca cacaggagaa
     1921 aaaccctata cctgtcatga gtgcggagac agcttctctc acagctccaa tcggattcgc
     1981 cacctgagaa cgcatacggg agagagaccc tataaatgtt ctgaatgtgg agaaagcttc
     2041 tctcggagtt cccgtcttat gagtcatcag agaactcaca caggttaccc aactttcttg
     2101 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2161 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2221 ggaaaggacg attaattcac ttaattgcat ttagt
//
