LOCUS       sequence-286623-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3360
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(890..914)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             922..963
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agcccggcaa ttatgccacc
       61 ttggatggag ccaaggatat cgaaggcttg ctgggagcgg gaggggggcg gaatctggtc
      121 gcccactccc ctctgaccag ccacccagcg gcgcctacgc tgatgcctgc tgtcaactat
      181 gcccccttgg atctgccagg ctcggcggag ccgccaaagc aatgccaccc atgccctggg
      241 gtgccccagg ggacgtcccc agctcccgtg ccttatggtt actttggagg cgggtactac
      301 tcctgccgag tgtcccggag ctcgctgaaa ccctgtgccc aggcagccac cctggccgcg
      361 taccccgcgg agactcccac ggccggggaa gagtacccca gccgccccac tgagtttgcc
      421 ttctatccgg gatatccggg aacctaccag cctatggcca gttacctgga cgtgtctgtg
      481 gtgcagactc tgggtgctcc tggagaaccg cgacatgact ccctgttgcc tgtggacagt
      541 taccagtctt gggctctcgc tggtggctgg aacagccaga tgtgttgcca gggagaacag
      601 aacccaccag gtcccttttg gaaggcagca tttgcagact ccagcgggca gcaccctcct
      661 gacgcctgcg cctttcgtcg cggccgcaag aaacgcattc cgtacagcaa ggggcagttg
      721 cgggagctgg agcgggagta tgcggctaac aagttcatca ccaaggacaa gaggcgcaag
      781 atctcggcag ccaccagcct ctcggagcgc cagattacca tctggtttca gaaccgccgg
      841 gtcaaagaga agaaggttct cgccaaggtg aagaacagcg ctacccctta cccaactttc
      901 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      961 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1021 tgtggaaagg acgaggcccc agtgcccggg ttcgttca
//
