LOCUS       sequence-286629-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3362
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(710..734)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             742..783
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agctgtatga gacatccccc
       61 tacttctacc aggaaccccg cttctatgat ggggaaaact acctgcctgt ccacctccag
      121 ggcttcgaac caccaggcta cgagcggacg gagctcaccc tgagccccga ggccccaggg
      181 ccccttgagg acaaggggct ggggaccccc gagcactgtc caggccagtg cctgccgtgg
      241 gcgtgtaagg tgtgtaagag gaagtcggtg tccgtggacc ggcggcgggc ggccacactg
      301 agggagaagc gcaggctcaa gaaggtgaat gaggccttcg aggccctgaa gagaagcacc
      361 ctgctcaacc ccaaccagcg gctgcccaag gtggagatcc tgcgcagtgc catccagtac
      421 atcgagcgcc tccaggccct gctcagctcc ctcaaccagg aggagcgtga cctccgctac
      481 cggggcgggg gcgggcccca gccaggggtg cccagcgaat gcagctctca cagcgcctcc
      541 tgcagtccag agtggggcag tgcactggag ttcagcgcca acccagggga tcatctgctc
      601 acggctgacc ctacagatgc ccacaacctg cactccctca cctccatcgt ggacagcatc
      661 acagtggaag atgtgtctgt ggccttccca gatgaaacca tgcccaactg cccaactttc
      721 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      781 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgattgctc cttaaattgt ggctacac
//
