LOCUS       sequence-286632-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3363
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1421..1445)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1453..1494
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg actattcgta cgacgaggac
       61 ctggacgagc tgtgccccgt gtgcggggac aaggtgtccg gctaccacta cggactgctc
      121 acgtgtgaga gctgcaaggg cttcttcaag cgcacggtgc agaacaacaa gcactacacg
      181 tgcaccgaga gccagagctg caagatcgac aagacgcagc gcaagcgctg tcccttctgc
      241 cgcttccaga aatgcctgac ggtggggatg cgcctggaag ccgtgcgcgc tgaccgtatg
      301 aggggtggcc ggaacaagtt tgggccgatg tacaagcggg accgggccct gaaacagcag
      361 aagaaggcac agattcgggc caatggcttc aagctggaga cagggccccc gatgggggtg
      421 cccccgccgc cccctcccgc accggactac gtgctgcctc ccagcctgca tgggcctgag
      481 cccaagggcc tggccgccgg tccacctgct gggccactgg gcgactttgg ggccccagca
      541 ctgcccatgg ccgtgcccgg tgcccacggg ccactggctg gctacctcta ccctgccttt
      601 cctggccgtg ccatcaagtc tgagtacccg gagccttatg ccagcccccc acagcctggg
      661 ctgccgtacg gctacccaga gcccttctct ggagggccca acgtgcctga gctcatcctg
      721 cagctgctgc agctggagcc ggatgaggac caggtgcggg cccgcatctt gggctgcctg
      781 caggagccca ccaaaagccg ccccgaccag ccggcggcct tcggcctcct gtgcagaatg
      841 gccgaccaga ccttcatctc catcgtggac tgggcacgca ggtgcatggt cttcaaggag
      901 ctggaggtgg ccgaccagat gacgctgctg cagaactgct ggagcgagct gctggtgttc
      961 gaccacatct accgccaggt ccagcacggc aaggagggca gcatcctgct ggtcaccggg
     1021 caggaggtgg agctgaccac agtggccacc caggcgggct cgctgctgca cagcctggtg
     1081 ttgcgggcgc aggagctggt gctgcagctg cttgcgctgc agctggaccg gcaggagttt
     1141 gtctgcctca agttcatcat cctcttcagc ctggatttga agttcctgaa taaccacatc
     1201 ctggtgaaag acgctcagga gaaggccaac gccgccctgc ttgactacac cctgtgccac
     1261 tacccgcact gcggggacaa attccagcag ctgctgctgt gcctggtgga ggtgcgggcc
     1321 ctgagcatgc aggccaagga gtacctgtac cacaagcacc tgggcaacga gatgccccgc
     1381 aacaacctgc tcatcgaaat gctgcaagcc aagcagactt gcccaacttt cttgtacaaa
     1441 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1501 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1561 gacgacaatg tgattcgagg ccactgctt
//
