LOCUS       sequence-286635-        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3364
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1289..1313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1321..1362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgtatcact aacagcagct
       61 gaaactctgg cccttcaggg tacacaggga caagagaaga tgatgatgat gggaccaaag
      121 gaagaggaac agtcttgtga gtatgagacc aggctacctg ggaaccactc taccagtcaa
      181 gagatcttcc gccaacgctt caggcatctc cgctaccagg agactcctgg tccccgggag
      241 gccttgagcc aactacgagt actctgctgt gagtggctga ggccagagaa acacacgaag
      301 gagcagatcc tggagttcct ggtgctggaa caattcttga ccatcctgcc tgaggagctc
      361 caatcctggg tgcggggaca tcaccctaag agtggagagg aggctgtgac tgtgctggag
      421 gatttagaga aaggacttga accagagccg caggtcccag gccctgcaca tggacctgca
      481 caggaagagc catgggagaa gaaggaatct ctgggagcag cccaggaagc actgagcatc
      541 cagctccagc ctaaggagac ccagcctttc ccaaagagtg aacaggtata tttacatttt
      601 ctgtcagttg ttacagaaga tggcccagag cccaaggaca aaggatcatt gccacaacca
      661 cccattactg aagtggaatc acaggtgttc tcagaaaaac ttgctactga cacctctaca
      721 tttgaagcta cctctgaggg taccttagaa ctgcagcaga gaaatcccaa agcggagaga
      781 ctgaggtggt cccctgccca ggaggaaagt ttcaggcaga tggttgtcat ccataaggaa
      841 attcccacag ggaagaaaga ccatgaatgt agtgaatgtg gtaaaacctt catttataac
      901 tcacatcttg ttgtccacca gagagttcat tctggagaga aaccctataa gtgtagtgac
      961 tgtgggaaaa ctttcaaaca gagctcaaac ctcggtcagc atcagagaat tcatacagga
     1021 gagaaaccct tcgaatgtaa tgaatgtggg aaggccttca gatggggtgc tcatcttgtt
     1081 cagcatcaga ggattcactc aggagagaag ccctatgagt gtaatgagtg tgggaaggcc
     1141 tttagtcaaa gctcatatct aagtcagcat cggagaattc acagtggaga gaaacctttt
     1201 atatgtaaag aatgtgggaa agcttatgga tggtgctcag agctcattag acatcggaga
     1261 gttcatgcca gaaaagagcc ttcccattgc ccaactttct tgtacaaagt ggttgatatc
     1321 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1381 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaatacttc
     1441 aaaaagaaat tgaaccg
//
