LOCUS       sequence-286638-        1622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3365
FEATURES             Location/Qualifiers
     source          1..1622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1454..1478)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1486..1527
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt taggaactgt gaagatggaa
       61 gggcatgaaa ccagcgactg gaacagctac tacgcagaca cgcaggaggc ctactcctcc
      121 gtcccggtca gcaacatgaa ctcaggcctg ggctccatga actccatgaa cacctacatg
      181 accatgaaca ccatgactac gagcggcaac atgaccccgg cgtccttcaa catgtcctat
      241 gccaacccgg gcctaggggc cggcctgagt cccggcgcag tagccggcat gccggggggc
      301 tcggcgggcg ccatgaacag catgactgcg gccggcgtga cggccatggg tacggcgctg
      361 agcccgagcg gcatgggcgc catgggtgcg cagcaggcgg cctccatgaa tggcctgggc
      421 ccctacgcgg ccgccatgaa cccgtgcatg agccccatgg cgtacgcgcc gtccaacctg
      481 ggccgcagcc gcgcgggcgg cggcggcgac gccaagacgt tcaagcgcag ctacccgcac
      541 gccaagccgc cctactcgta catctcgctc atcaccatgg ccatccagca ggcgcccagc
      601 aagatgctca cgctgagcga gatctaccag tggatcatgg acctcttccc ctattaccgg
      661 cagaaccagc agcgctggca gaactccatc cgccactcgc tgtccttcaa tgactgcttc
      721 gtcaaggtgg cacgctcccc ggacaagccg ggcaagggct cctactggac gctgcacccg
      781 gactccggca acatgttcga gaacggctgc tacttgcgcc gccagaagcg cttcaagtgc
      841 gagaagcagc cgggggccgg cggcgggggc gggagcggaa gcgggggcag cggcgccaag
      901 ggcggccctg agagccgcaa ggacccctct ggcgcctcta accccagcgc cgactcgccc
      961 ctccatcggg gtgtgcacgg gaagaccggc cagctagagg gcgcgccggc ccccgggccc
     1021 gccgccagcc cccagactct ggaccacagt ggggcgacgg cgacaggggg cgcctcggag
     1081 ttgaagactc cagcctcctc aactgcgccc cccataagct ccgggcccgg ggcgctggcc
     1141 tctgtgcccg cctctcaccc ggcacacggc ttggcacccc acgagtccca gctgcacctg
     1201 aaaggggacc cccactactc cttcaaccac ccgttctcca tcaacaacct catgtcctcc
     1261 tcggagcagc agcataagct ggacttcaag gcatacgaac aggcactgca atactcgcct
     1321 tacggctcta cgttgcccgc cagcctgcct ctaggcagcg cctcggtgac caccaggagc
     1381 cccatcgagc cctcagccct ggagccggcg tactaccaag gtgtgtattc cagacccgtc
     1441 ctaaacactt cctacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1501 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1561 tcgatttctt ggctttatat atcttgtgga aaggacgata tgtagcacct gagtcttcct
     1621 cg
//
