LOCUS       sequence-286641-        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3366
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(892..903)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1088..1112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1120..1161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tgggctcagt gaagatggag
       61 gcccatgacc tggccgagtg gagctactac ccggaggcgg gcgaggtcta ctcgccggtg
      121 accccagtgc ccaccatggc ccccctcaac tcctacatga ccctgaatcc tctaagctct
      181 ccctatcccc ctggggggct ccctgcctcc ccactgccct caggacccct ggcaccccca
      241 gcacctgcag cccccctggg gcccactttc ccaggcctgg gtgtcagcgg tggcagcagc
      301 agctccgggt acggggcccc gggtcctggg ctggtgcacg ggaaggagat gccgaagggg
      361 tatcggcggc ccctggcaca cgccaagcca ccgtattcct atatctcact catcaccatg
      421 gccatccagc aggcgccggg caagatgctg accttgagtg aaatctacca gtggatcatg
      481 gacctcttcc cttactaccg ggagaatcag cagcgctggc agaactccat tcgccactcg
      541 ctgtctttca acgactgctt cgtcaaggtg gcgcgttccc cagacaagcc tggcaagggc
      601 tcctactggg ccctacaccc cagctcaggg aacatgtttg agaatggctg ctacctgcgc
      661 cgccagaaac gcttcaagct ggaggagaag gtgaaaaaag ggggcagcgg ggctgccacc
      721 accaccagga acgggacagg gtctgctgcc tcgaccacca cccccgcggc cacagtcacc
      781 tccccgcccc agcccccgcc tccagcccct gagcctgagg cccagggcgg ggaagatgtg
      841 ggggctctgg actgtggctc acccgcttcc tccacaccct atttcactgg cctggagctc
      901 ccaggggagc tgaagctgga cgcgccctac aacttcaacc accctttctc catcaacaac
      961 ctaatgtcag aacagacacc agcacctccc aaactggacg tggggtttgg gggctacggg
     1021 gctgaaggtg gggagcctgg agtctactac cagggcctct attcccgctc tttgcttaat
     1081 gcatcctacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1141 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1201 tcttggcttt atatatcttg tggaaaggac gacacgaagc aggaactcga taacag
//
