LOCUS       sequence-286644-        2891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3367
FEATURES             Location/Qualifiers
     source          1..2891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             457..480
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    complement(2723..2747)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2755..2796
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga aaatcggcag tgggttcctg
       61 agtggcggcg gaggtaccgg cagtagcggt ggtagcggct ccggcggcgg tggtagtggc
      121 ggcggcggcg gcggcggcag cagcggcagg agggcagaga tggaacccac ctttccccag
      181 ggtatggtta tgttcaacca ccgtcttccc ccggtcacca gcttcacccg gccggcgggg
      241 tcggccgccc ctcccccgca atgcgtgtta tcctcctcta cctccgcagc cccggccgct
      301 gagccccccc ctccgccagc cccggacatg actttcaaga aggagccggc ggcgtcagcc
      361 gcggccttcc cctcgcagag gacctcctgg gggttcttgc agtctttggt tagcatcaaa
      421 caggagaaac ccgcggatcc tgaggagcag cagtcccacc accaccatca ccaccaccac
      481 tatggggggc tgttcgctgg agctgaagag aggtctccag gcctaggagg cggtgaaggg
      541 gggagtcacg gcgtcatcca ggacctcagt attctccacc agcatgtcca gcagcaacca
      601 gcccagcacc accgtgacgt attactcagc agcagtagca ggactgatga ccaccatggc
      661 actgaggagc caaagcagga cactaatgtc aaaaaggcaa aaaggccaaa gccagaatct
      721 cagggaatca aagccaagag gaagccaagt gcatcttcca aaccttcttt ggttggagat
      781 ggagaaggtg ccatcctctc cccaagtcag aaacctcata tctgtgatca ctgtagtgct
      841 gctttccgaa gctcctatca cctgcggaga catgtcctca ttcatacagg agaaagacct
      901 ttccagtgca gccagtgtag tatgggtttc attcagaaat acctactaca gagacatgag
      961 aaaattcata gtagagagaa gccatttgga tgtgatcagt gcagcatgaa gtttattcag
     1021 aagtaccata tggagagaca caagaggaca catagtggag aaaagccata taagtgtgac
     1081 acttgccaac agtatttttc aaggactgat agattgttga agcacaggcg cacatgtggt
     1141 gaagtcatag ttaaaggagc cactagtgca gaacctgggt catcaaacca taccaatatg
     1201 ggtaatctgg ctgtgttgtc tcagggaaat acaagttctt caaggagaaa aacaaagtca
     1261 aaaagcatag ctattgaaaa taaggaacag aagaccggta aaacaaatga atcacaaatt
     1321 tcaaataata taaacatgca gagttactca gtagaaatgc ctaccgtgtc ttccagtgga
     1381 ggcataattg gcactggaat agatgaactg cagaagaggg tgccaaaatt gatctttaag
     1441 aaaggaagca gaaagaatac agataaaaac taccttaact ttgtgtcacc attaccagac
     1501 atagtaggac agaaatcctt gtctggaaaa ccaagtggct cacttggcat agtatcaaat
     1561 aatagtgtgg agaccattgg tcttctccaa agtacaagtg gcaaacaagg tcagataagt
     1621 agtaattatg atgatgccat gcagttttca aagaaaagaa gatatttacc aactgccagc
     1681 agcaacagtg ccttttctat aaacgtagga cacatggtct cccaacagtc tgtcattcag
     1741 tctgcaggtg tcagtgtttt ggacaatgag gcaccattgt cacttattga ctcctcagct
     1801 ctaaatgctg aaattaaatc ttgtcatgac aagtctggaa ttcctgatga ggttttacaa
     1861 agtattttgg atcaatactc caacaaatca gaaagccaga aagaggatcc tttcaatatt
     1921 gcagaaccac gagtggattt acacacctca ggagaacact cagaattggt tcaagaagaa
     1981 aatttgagcc caggcaccca aacaccttca aatgataaag caagtatgtt gcaagaatac
     2041 tccaaatacc tccaacaggc ttttgaaaaa tccactaatg caagttttac tcttggacac
     2101 ggtttccaat ttgtcagttt gtcttcacct ctccacaacc acactttgtt tccagaaaaa
     2161 caaatataca ctacgtctcc tttggagtgt ggtttcggcc aatctgttac ctcagtgttg
     2221 ccatcttcat tgccaaagcc tccttttggg atgttgtttg gatctcagcc aggtctttat
     2281 ttgtctgctt tggatgctac acatcagcag ttgacacctt cccaggagct ggatgatctg
     2341 atagattctc agaagaactt agagacttca tcagccttcc agtcctcatc tcagaaattg
     2401 actagccaga aggaacagaa aaacttagag tcttcaacag gctttcagat tccatctcag
     2461 gagttagcta gccagataga tcctcagaaa gacatagagc ctagaacaac gtatcagatt
     2521 gagaactttg cacaagcgtt tggttctcag tttaagtcgg gcagcagggt gccaatgacc
     2581 tttatcacta actctaatgg agaagtggac catagagtaa ggacttcagt gtcagatttc
     2641 tcagggtata caaatatgat gtctgatgta agtgagccat gtagtacaag agtaaagaca
     2701 cccaccagcc agagttacag gtacccaact ttcttgtaca aagtggttga tatcggtaag
     2761 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     2821 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaatg acccgatgcc
     2881 ggcctcaggc c
//
