LOCUS       sequence-286650-        1406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3369
FEATURES             Location/Qualifiers
     source          1..1406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1238..1262)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1270..1311
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ctactaaaaa tttagaaacc
       61 aaagtcaccg ttacttcatc cccaatccga ggagcaggag atggaatgga aactgaggaa
      121 ccacctaaat ctgttgaagt tacctccgga gtccaatcta gaaagcatca tagtcttcag
      181 agtccatgga agaaagcagt tccatcagag agcccaggag ttcttcagct agggaaaatg
      241 ctcactgaaa aagcaatgga agttaaagct gtaagaatat tagttcccaa agctgctata
      301 actcatgata tccccaacaa aaatacaaag gttaagtctc tgggacatca taaaggagaa
      361 ttccttggtc agtcagaggg agttatagaa cctaataagg aactctcaga ggtaaagaat
      421 gtattggaaa agctcaagaa ttctgaaaga aggttactac aggacaaaga aggtctttca
      481 aaccagctcc gtgtacagac agaggtaaat cgtgagttaa aaaagttact ggtggcttct
      541 gttggggatg atcttcagta tcactttgaa cgtctagccc gtgagaaaaa tcagcttatt
      601 ttagaaaatg aagccctagg tcgaaacaca gctcagcttt ctgaacagtt agaacgtatg
      661 tcaatacagt gtgatgtatg gcgaagtaaa ttccttgcaa gcagggtaat ggcagatgag
      721 ttaaccaact caagagcagc tttacagcgt caaaaccgtg atgcacacgg ggctatacaa
      781 gatctcctaa gtgaacggga acagtttcgt caagaaatga tagctaccca gaaattattg
      841 gaggagctct tagtttcctt gcaatgggga agagagcaaa cttactcccc tagtgtacaa
      901 ccccacagca cagcagagct agcattaaca aatcacaagt tggcaaaagc agtaaattct
      961 catcttctgg gaaatgttgg cattaacaat caaaaaaaga ttccatcaac agttgaattc
     1021 tgcagcaccc cagctgagaa aatggctgaa acggttctaa gaattttaga tccagttacc
     1081 tgcaaagaga gttcacctga taatccattt tttgagtctt caccaaccac cttacttgct
     1141 acaaagaaaa atattggacg atttcatccc tatactagat atgaaaatat aactttcaat
     1201 tgctgcaatc actgccgggg agaactgatt gccctttacc caactttctt gtacaaagtg
     1261 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1321 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1381 gacattcctg ttgtccctcc ttaacg
//
