LOCUS       sequence-286653-        2285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3370
FEATURES             Location/Qualifiers
     source          1..2285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2117..2141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2149..2190
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgaagag gtcgcgcagt
       61 gaggatgagg atgatgacct tcagtatgcc gatcatgatt atgaagtacc acaacaaaaa
      121 ggactgaaga aactctggaa cagagtaaaa tggacaaggg acgaggatga taaattaaag
      181 aagttggttg aacaacatgg aactgatgat tggactctaa ttgctagtca tcttcaaaat
      241 cgctctgatt ttcagtgcca gcatcgatgg cagaaagttt taaatcctga attgataaag
      301 ggtccttgga ctaaagaaga agatcagagg gttattgaat tagttcagaa atatgggcca
      361 aaaagatggt ctttaattgc aaaacattta aaaggaagaa taggcaagca gtgtagagaa
      421 agatggcata atcatctgaa tcctgaggta aagaaatctt cctggacaga agaggaggac
      481 aggatcatct atgaagcaca taagcggttg ggaaatcgtt gggcagaaat tgccaaacta
      541 cttccaggaa ggactgataa ttctatcaaa aatcattgga attctactat gcgaagaaaa
      601 gtggaacagg agggctattt acaagatgga ataaaatcag aacgatcttc atctaaactt
      661 caacacaaac cttgtgcagc tatggatcat atgcaaaccc agaatcagtt ttacatacct
      721 gttcagatcc ctgggtatca gtatgtgtca cctgaaggca attgtataga acatgttcag
      781 cctacttctg cctttattca gcaacccttc attgatgaag atcctgataa ggaaaagaaa
      841 ataaaggaac ttgagatgct tcttatgtca gctgagaatg aagttagaag aaagcgaatt
      901 ccatcacagc ctggaagttt ttctagctgg tctggtagtt tcctcatgga tgataacatg
      961 tctaatactc taaatagcct tgacgagcac actagtgagt tttacagtat ggatgaaaat
     1021 cagcctgtgt ctgctcagca gaattcaccc acaaagttcc tggccgtgga ggcaaacgct
     1081 gtgttatcct ctttgcagac catcccagaa tttgcagaga ctctagaact tattgaatct
     1141 gatcctgtag catggagtga cgttaccagt tttgatattt ctgatgctgc tgcttctcct
     1201 atcaaatcca ccccagttaa attaatgaga attcagcaca atgaaggagc catggaatgc
     1261 caatttaacg tcagtcttgt acttgaaggg aaaaaaaaca cttgtaatgg tggcaacagt
     1321 gaagctgttc ctttaacatc cccaaatata gccaagttta gcactccacc agccatcctc
     1381 agaaagaaga gaaaaatgcg agtgggtcat tccccaggca gcgaacttag ggatggctca
     1441 ttgaacgatg gtggtaatat ggcgctaaaa catacaccac tgaaaacact accattttct
     1501 ccttcacagt ttttcaacac atgtcctggt aatgaacaac ttaatataga aaatccttca
     1561 tttacatcaa cccctatttg tgggcagaaa gctctcatta caactcctct tcataaggaa
     1621 acaactccca aagatcaaaa ggaaaatgta gggtttagaa cacctactat tagaagatct
     1681 atactgggta ccacaccaag aactcctact ccttttaaga atgcgcttgc tgctcaggag
     1741 aaaaaatatg gacctcttaa aattgtgtcc cagccacttg ctttcttgga agaagatatt
     1801 cgggaagttt taaaagaaga aactggaaca gacctattcc tcaaagagga agatgaacct
     1861 gcttacaaaa gctgcaaaca agagaatacc gcttctggga agaaagtcag aaaatcacta
     1921 gtcttagata attgggaaaa agaagaatca ggcactcaac tgttgactga agacatttca
     1981 gacatgcagt caaattgtga atgggaaaca gtggtttatg ggaagacaga agaccaactt
     2041 attatgactg aacaagcaag aagatatctg agtacttaca cagctaccag tagtacttca
     2101 agagctctca tactgttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2161 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2221 atttcgattt cttggcttta tatatcttgt ggaaaggacg atcacacaca tgctcgctga
     2281 ctgtg
//
