LOCUS       sequence-286656-        2282 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3371
FEATURES             Location/Qualifiers
     source          1..2282
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2114..2138)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2146..2187
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcgaagag gtcgcgcagt
       61 gaggatgagg atgatgacct tcagtatgcc gatcatgatt atgaagtacc acaacaaaaa
      121 ggactgaaga aactctggaa cagagtaaaa tggacaaggg acgaggatga taaattaaag
      181 aagttggttg aacaacatgg aactgatgat tggactctaa ttgctagtca tcttcaaaat
      241 cgctctgatt ttcagtgcca gcatcgatgg cagaaagttt taaatcctga attgataaag
      301 ggtccttgga ctaaagaaga agatcagagg gttattgaat tagttcagaa atatgggcca
      361 aaaagatggt ctttaattgc aaaacattta aaaggaagaa taggcaagca gtgtagagaa
      421 agatggcata atcatctgaa tcctgaggta aagaaatctt cctggacaga agaggaggac
      481 aggatcatct atgaagcaca taagcggttg ggaaatcgtt gggcagaaat tgccaaacta
      541 cttccaggaa ggactgataa ttctatcaaa aatcattgga attctactat gcgaagaaaa
      601 gtggaacagg agggctattt acaagatgga ataaaatcag aacgatcttc atctaaactt
      661 caacacaaac cttgtgcagc tatggatcat atgcaaaccc agaatcagtt ttacatacct
      721 gttcagatcc ctgggtatca gtatgtgtca cctgaaggca attgtataga acatgttcag
      781 cctacttctg cctttattca gcaacccttc attgatgaag atcctgataa ggaaaagaaa
      841 ataaaggaac ttgagatgct tcttatgtca gctgagaatg aagttagaag aaagcgaatt
      901 ccatcacctg gaagtttttc tagctggtct ggtagtttcc tcatggatga taacatgtct
      961 aatactctaa atagccttga cgagcacact agtgagtttt acagtatgga tgaaaatcag
     1021 cctgtgtctg ctcagcagaa ttcacccaca aagttcctgg ccgtggaggc aaacgctgtg
     1081 ttatcctctt tgcagaccat cccagaattt gcagagactc tagaacttat tgaatctgat
     1141 cctgtagcat ggagtgacgt taccagtttt gatatttctg atgctgctgc ttctcctatc
     1201 aaatccaccc cagttaaatt aatgagaatt cagcacaatg aaggagccat ggaatgccaa
     1261 tttaacgtca gtcttgtact tgaagggaaa aaaaacactt gtaatggtgg caacagtgaa
     1321 gctgttcctt taacatcccc aaatatagcc aagtttagca ctccaccagc catcctcaga
     1381 aagaagagaa aaatgcgagt gggtcattcc ccaggcagcg aacttaggga tggctcattg
     1441 aacgatggtg gtaatatggc gctaaaacat acaccactga aaacactacc attttctcct
     1501 tcacagtttt tcaacacatg tcctggtaat gaacaactta atatagaaaa tccttcattt
     1561 acatcaaccc ctatttgtgg gcagaaagct ctcattacaa ctcctcttca taaggaaaca
     1621 actcccaaag atcaaaagga aaatgtaggg tttagaacac ctactattag aagatctata
     1681 ctgggtacca caccaagaac tcctactcct tttaagaatg cgcttgctgc tcaggagaaa
     1741 aaatatggac ctcttaaaat tgtgtcccag ccacttgctt tcttggaaga agatattcgg
     1801 gaagttttaa aagaagaaac tggaacagac ctattcctca aagaggaaga tgaacctgct
     1861 tacaaaagct gcaaacaaga gaataccgct tctgggaaga aagtcagaaa atcactagtc
     1921 ttagataatt gggaaaaaga agaatcaggc actcaactgt tgactgaaga catttcagac
     1981 atgcagtcaa attgtgaatg ggaaacagtg gtttatggga agacagaaga ccaacttatt
     2041 atgactgaac aagcaagaag atatctgagt acttacacag ctaccagtag tacttcaaga
     2101 gctctcatac tgttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     2161 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     2221 tcgatttctt ggctttatat atcttgtgga aaggacgatg gcgccctggt tgcgactcta
     2281 ct
//
