LOCUS       sequence-286659-        2429 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3372
FEATURES             Location/Qualifiers
     source          1..2429
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2261..2285)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2293..2334
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggccaccgc taacagcatc
       61 atcgtgctgg atgatgatga cgaagatgaa gcagctgctc agccagggcc ctcccaccca
      121 ctccccaatg cggcctcacc tggggcagaa gcccctagct cctctgagcc tcatggggcc
      181 agaggaagca gtagttcggg cggcaagaaa tgctacaagc tggagaatga gaagctgttc
      241 gaagagttcc ttgaactttg taagatgcag acagcagacc accctgaggt ggtcccattc
      301 ctctataacc ggcagcaacg tgcccactct ctgtttttgg cctcggcgga gttctgcaac
      361 atcctctcta gggtcctgtc tcgggcccgg agccggccag ccaagctcta tgtctacatc
      421 aatgagctct gcactgttct caaggcccac tcagccaaaa agaagctgaa cttggcccct
      481 gccgccacca cctccaatga gccctctggg aataaccctc ccacacacct ctccttggac
      541 cccacaaatg ctgaaaacac tgcctctcag tctccaagga cccgtggttc ccggcggcag
      601 atccagcgtt tggagcagct gctggcgctc tatgtggcag agatccggcg gctgcaggaa
      661 aaggagttgg atctctcaga attggatgac ccagactccg catacctgca ggaggcacgg
      721 ttgaagcgta agctgatccg cctctttggg cgactatgtg agctgaaaga ctgctcttca
      781 ctgaccggcc gtgtcataga gcagcgcatc ccctaccgtg gcacccgcta cccagaggtt
      841 aacaggcgca ttgagcggct catcaacaag ccagggcctg ataccttccc tgactatggg
      901 gatgtgcttc gggctgtaga gaaggcagct gcccgacaca gccttggcct cccccgacag
      961 cagctccagc tcatggctca ggatgccttc cgagatgtgg gcatcaggtt acaggagcga
     1021 cgtcacctcg atctcatcta caactttggc tgccacctca cagatgacta taggccaggc
     1081 gttgaccctg cactatcaga tcctgtgttg gcccggcgcc ttcgggaaaa ccggagtttg
     1141 gccatgagtc ggctggatga ggtcatctcc aaatatgcaa tgttgcaaga caaaagtgag
     1201 gagggcgaga gaaaaaagag aagagctcgg ctccaaggca cctcttccca ctctgcagac
     1261 acccccgaag cctccttgga ttctggtgag ggccctagtg gaatggcatc ccaggggtgc
     1321 ccttctgcct ccagagctga gacagatgac gaagacgatg aggagagtga tgaggaagag
     1381 gaggaggagg aggaagaaga agaggaggag gccacagatt ctgaagagga ggaggatctg
     1441 gaacagatgc aggagggtca ggaggatgat gaagaggagg acgaagagga agaagcagca
     1501 gcaggtaaag atggagacaa gagccccatg tcctcactac agatctccaa tgaaaagaac
     1561 ctggaacctg gcaaacagat cagcagatct tcaggggagc agcaaaacaa aggacgcata
     1621 gtgtcaccat cgttactgtc agaagaaccc ctggccccct ccagcataga tgctgaaagc
     1681 aatggagaac agcctgagga gctgaccctg gaggaagaaa gccctgtgtc tcagctcttt
     1741 gagctagaga ttgaagcttt gcccctggat accccttcct ctgtggagac ggacatttcc
     1801 tcttccagga agcaatcaga ggagcccttc accactgtct tagagaatgg agcaggcatg
     1861 gtctcttcta cttccttcaa tggaggcgtc tctcctcaca actggggaga ttctggtccc
     1921 ccctgcaaaa aatctcggaa ggagaagaag caaacaggat cagggccatt aggaaacagc
     1981 tatgtggaaa ggcaaaggtc agtgcatgag aagaatggga aaaagatatg taccctgccc
     2041 agcccacctt cccccttggc ttccttggcc ccagttgctg attcctccac gagggtggac
     2101 tctcccagcc atggcctggt gaccagctcc ctctgcatcc cttctccagc ccggctgtcc
     2161 caaacccccc attcacagcc tcctcggcct ggtacttgca agacaagtgt ggccacacaa
     2221 tgcgatccag aagagatcat cgtgctctca gactctgatt tgccaacttt cttgtacaaa
     2281 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     2341 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2401 gacgacaccc ctcagctcag tcgccgatc
//
