LOCUS       sequence-28666-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell
            A, Johnson GL, Hahn KM, Danuser G
  TITLE     Coordination of Rho GTPase activities during cell protrusion.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):99-103.
  PUBMED    19693013
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):99-103."
COMMENT     Sequence Label: pCyPet-Rac1(T17N)
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     661..680
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caaattaaga acacatctgt ttgcggatag gatagggggc gtaatctgtc ataatcttct
       61 tgtccagctg tatcccataa gcccagattc accggttttc catctaccat aacattggca
      121 gaataattgt caaagacagt agggatatat tctccaggaa atgcattggt tgtgtaactg
      181 atcagtaggc aatttttacc tacagctccg tctcccacca ccacacactt gatggcctgc
      241 ggggatccga gctcagatct gagtccggac ttgtacagct cgtccatgcc gtgggtgatg
      301 ccggcggcgg tcacgaactc cagcagcacc atgtggtccc gcttctcgtt ggggtccttg
      361 ctcagggcgc tctgggtgct caggtagtgg ttgtcgggca ggatcacggg gccgtcgccg
      421 atgggggtgt tctgctggta gtggtcggcc agctgcacgc tgccgtcggt gatgttgtgc
      481 cgggccttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat gtacacgttg
      541 tggctgatgt agttgtactc cagcttgtgg cccaggatgt tgccgtcctc cttgaagtcg
      601 atgcccttca gctcgatccg gttcaccagg gtgtcgccct cgaacttcac ctcggcccgg
      661 gtcttgtagt tgccgtcgtc cttgaagaag atggtccgct cctgcacgta gnc
//
