LOCUS       sequence-286662-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3373
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(986..1010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1018..1059
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gagctcatgc tggacacaaa
       61 cgatctgagt gtggtgggga atggagagag acgccccgta aacagaaaca acatgggaaa
      121 gcctccattt cccccagtag tggtgcacgg cgcacagtaa caccaactcg aaagagacct
      181 tatgaatgca aggtgtgcgg gaaagccttt aattctccca atttatttca aatccatcaa
      241 agaactcaca ctggaaagag gtcctataaa tgtagggaaa tagtgagagc cttcacagtt
      301 tccagtttct ttcgaaaaca tggaaaaatg catactggag aaaaacgcta tgaatgtaaa
      361 tactgtggaa aacctatcga ttatcccagt ttatttcaaa ttcatgttag aactcacact
      421 ggagaaaaac cttacaaatg taaacaatgt ggtaaagcct tcatttccgc aggttacctt
      481 cggacacatg aaatcagatc tcacgcgctg gagaaatccc accaatgtca ggaatgtggg
      541 aaaaaactca gttgttccag ttcccttcac agacatgaaa gaactcatag tggaggaaaa
      601 ctctacgaat gtcaaaaatg tgccaaagtc tttagatgtc ccacgtccct tcaagcacat
      661 gaaagagctc acactggaga aagaccttat gaatgtaata aatgtggtaa aaccttcaat
      721 tatcccagtt gttttcgaag acataaaaaa actcatagtg gagaaaagcc atatgaatgt
      781 acaaggtgtg gtaaagcctt tgggtggtgc agttccctcc gaagacatga aatgactcac
      841 actggagaaa aaccctttga ttgtaaacag tgtggtaaag tctttacttt ttcaaattac
      901 cttagacttc atgaaagaac tcatttggcc gggcgtagcc agtgctttgg caggaggcag
      961 ggggatcacc tgagcccagg agtttgccca actttcttgt acaaagtggt tgatatcggt
     1021 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1081 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aattcccatt
     1141 gtgattgggc ctgc
//
