LOCUS       sequence-286665-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3374
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             685..693
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(908..932)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             940..981
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gcgatggggg cgccgagcgc
       61 gaccggggcc ccgcgcgccg ggcggagtct ggtggcggcg gtgggcgctg cggagaccgc
      121 agcggagcgg gggacttgcg agctgatggc ggtggccaca gcccaacgga ggtggccggg
      181 acctcagcct ccagtcccgc aggctccagg gagagtggag ccgacagcga cgggcagccc
      241 gggcccggcg aggcagacca ctgccgccgc atactggtgc gagatgccaa agggacaatt
      301 cgggaaattg tcctgcctaa gggcctggac ctggaccggc ccaagcggac acgtacatcc
      361 ttcactgccg agcagctgta ccgcctggag atggagttcc agcgctgcca gtatgtggtg
      421 ggccgcgagc gcactgagct ggcccgccag ctgaacctct ccgagaccca ggtgaaggtc
      481 tggttccaga accgccgcac caagcagaag aaagaccaga gcagagacct ggagaagcgg
      541 gcgtcctcct cagcctccga ggcctttgcc acctccaaca ttctgcggct gctggagcag
      601 ggccggctgc tctctgtgcc cagggcccct agcctcctgg cgctgacccc tagcctgcca
      661 ggcctacctg ccagccacag gggcacctcc ttaggtgacc ccaggaactc ctccccacgc
      721 ctcaacccgc tgtcctcggc ctcagcgtcc cccccactgc cgccccctct gccagctgtc
      781 tgcttttcct cggccccgct cctggatctg cctgccggct acgaactggg ttcctcggcc
      841 ttcgagccat acagctggct agaacggaaa gtgggcagcg ccagcagctg caagaaagct
      901 aacacttacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      961 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1021 tcttggcttt atatatcttg tggaaaggac gatctaactc ttccacctcc agacaa
//
