LOCUS       sequence-286668-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3375
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(749..773)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             781..822
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cggacagtga tctaggtgag
       61 gacgaaggcc tcctctccct ggcgggcaaa aggaagcgca gggggaacct gcccaaggag
      121 tcggtgaaga tcctccggga ctggctgtac ttgcaccgct acaacgccta cccctcagag
      181 caggagaagc tgagcctttc tggacagacc aacctgtcag tgctgcaaat atgtaactgg
      241 ttcatcaatg cccggcggcg gcttctccca gacatgcttc ggaaggatgg caaagaccct
      301 aatcagttta ccatttcccg ccgcgggggt aaggcctcag atgtggccct cccccgtggc
      361 agcagcccct cagtgctggc tgtgtctgtc ccagccccca ccaatgtgct ctccctgtct
      421 gtgtgctcca tgccgcttca ctcaggccag ggggaaaagc cagcagcccc tttcccacgt
      481 ggggagctgg agtctcccaa gcccctggtg acccctggta gcacacttac tctgctgacc
      541 agggctgagg ctggaagccc cacaggtgga ctcttcaaca cgccaccacc cacaccccca
      601 gagcaggaca aagaggactt cagcagcttc cagctgctgg tggaggtggc gctacagagg
      661 gctgctgaga tggagcttca gaagcagcag gacccatcac tcccattact gcacactccc
      721 atccctttag tctctgaaaa tccccagtac ccaactttct tgtacaaagt ggttgatatc
      781 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaactgggt
      901 cacagtagtg attaccc
//
