LOCUS       sequence-286671-        1784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3376
FEATURES             Location/Qualifiers
     source          1..1784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1616..1640)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1648..1689
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acgtaagatt ttatccacct
       61 ccagcccagc ccgccgctgc gcccgacgct ccctgtctgg gaccttctcc ctgcctggac
      121 ccctactatt gcaacaagtt tgacggtgag aacatgtata tgagcatgac agagccgagc
      181 caggactatg tgccagccag ccagtcctac cctggtccaa gcctggaaag tgaagacttc
      241 aacattccac caattactcc tccttccctc ccagaccact cgctggtgca cctgaatgaa
      301 gttgagtctg gttaccattc tctgtgtcac cccatgaacc ataatggcct gctaccattt
      361 catccacaaa acatggacct ccctgaaatc acagtctcca atatgctggg ccaggatgga
      421 acactgcttt ctaattccat ttctgtgatg ccagatatac gaaacccaga aggaactcag
      481 tacagttccc atcctcagat ggcagccatg agaccaaggg gccagcctgc agacatcagg
      541 cagcagccag gaatgatgcc acatggccag ctgactacca ttaaccagtc acagctaagt
      601 gctcaacttg gtttgaatat gggaggaagc aatgttcccc acaactcacc atctccacct
      661 ggaagcaagt ctgcaactcc ttcaccatcc agttcagtgc atgaagatga aggcgatgat
      721 acctctaaga tcaatggtgg agagaagcgg cctgcctctg atatggggaa aaaaccaaaa
      781 actcccaaaa agaagaagaa gaaggatccc aatgagcccc agaagcctgt gtctgcctat
      841 gcgttattct ttcgtgatac tcaggccgcc atcaagggcc aaaatccaaa cgctaccttt
      901 ggcgaagtct ctaaaattgt ggcttcaatg tgggacggtt taggagaaga gcaaaaacag
      961 gtctataaaa agaaaaccga ggctgcgaag aaggagtacc tgaagcaact cgcagcatac
     1021 agagccagcc ttgtatccaa gagctacagt gaacctgttg acgtgaagac atctcaacct
     1081 cctcagctga tcaattcgaa gccgtcggtg ttccatgggc ccagccaggc ccactcggcc
     1141 ctgtacctaa gttcccacta tcaccaacaa ccgggaatga atcctcacct aactgccatg
     1201 catcctagtc tccccaggaa catagccccc aagccgaata accaaatgcc agtgactgtc
     1261 tctatagcaa acatggctgt gtcccctcct cctcccctcc agatcagccc gcctcttcac
     1321 cagcatctca acatgcagca gcaccagccg ctcaccatgc agcagcccct tgggaaccag
     1381 ctccccatgc aggtccagtc tgccttacac tcacccacca tgcagcaagg atttactctt
     1441 caacccgact atcagactat tatcaatcct acatctacag ctgcacaagt tgtcacccag
     1501 gcaatggagt atgtgcgttc ggggtgcaga aatcctcccc cacaaccggt ggactggaat
     1561 aacgactact gcagtagtgg gggcatgcag agggacaaag cactgtacct tacttgccca
     1621 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1681 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1741 atatcttgtg gaaaggacga tagccgccgt tacgcctatt ctca
//
